Listar Departamento de Sanidad Animal por autor "fb585e21-8eed-4d0f-bd0b-f1689525d0d7"
Mostrando ítems 1-17 de 17
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A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep
Molina Hernández, Verónica; Ruiz-Campillo, María Teresa; Martínez-Moreno, Francisco Javier; Buffoni Perazzo, Leandro; Martínez-Moreno, Álvaro; Zafra Leva, Rafael; Escamilla, Alejandro; Pérez Caballero, Raúl; Pérez Arévalo, José (MDPI, 2021)Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the ... -
Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
Escamilla, Alejandro; Pérez Caballero, Raúl; Zafra Leva, Rafael; Bautista, M.J.; Pacheco, I.L.; Ruiz, M.Teresa; Martínez Cruz, Setefilla; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Elsevier, 2017)Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present ... -
Caracterización de macrófagos peritoneales de ovejas en fases tempranas de infección experimental con fasciola hepática
Zafra Leva, Rafael; Pacheco, I.L.; Escamilla, Alejandro; Caballero, R.; Buffoni Perazzo, Leandro; Molina Hernández, Verónica; Martínez-Moreno, Álvaro; Pérez, José; Ruiz-Campillo, María Teresa (Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas, 2016) -
Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica
Ruiz-Campillo, María Teresa; Molina-Hernández, Verónica; Bautista, María José; Pacheco, I.L.; Zafra Leva, Rafael; Buffoni Perazzo, Leandro; Martínez-Moreno, Francisco Javier; Martínez Moreno, Álvaro; Pérez, José (Springer, 2020)Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment ... -
Distribución de linfocitos FOXP3 reguladores en hígado y nódulos linfáticos de ovejas y cabras infectadas con fasciola hepática
Escamilla, Alejandro; Zafra Leva, Rafael; Bautista Pérez, María José; Pacheco, I.L.; Ruiz-Campillo, María Teresa; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas, 2016) -
Distribution of Foxp3+ T cells in the liver and hepatic lymph nodes of goats and sheep experimentally infected with Fasciola hepatica
Escamilla, Alejandro; Zafra Leva, Rafael; Pérez, José; McNeilly, T.N.; Pacheco, I.L.; Buffoni Perazzo, Leandro; Martínez-Moreno, Francisco Javier; Molina Hernández, Verónica; Martínez-Moreno, Álvaro (Elsevier, 2016)Foxp3 regulatory T cells (Tregs) are now considered to play a key role in modulation of immune responses during parasitic helminth infections. Immunomodulation is a key factor in Fasciola hepatica infection; however, the ... -
Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep
Zafra Leva, Rafael; Buffoni Perazzo, Leandro; Pérez Caballero, Raúl; Molina-Hernández, Verónica; Ruiz-Campillo, María Teresa; Pérez, José; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier (Springer, 2021)In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants ... -
Evaluation of Th1/Th2, regulatory cytokines and transcriptional factor FoxP3 in sheep immunized with a partially protective and non-protective vaccine and challenged with Fasciola hepatica
Ruiz-Campillo, María Teresa; Pacheco, I.L.; Abril, Nieves; Bautista, M.J.; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Buffoni Perazzo, Leandro; Pérez, J.; Molina-Hernández, Verónica; Zafra Leva, Rafael (BMC (Springer), 2024)Gene expression for Th1/Th2 cytokines (IL-4 and IFN-ɣ), regulatory cytokines (TGF-β and IL-10) and the transcriptional factor FoxP3 was analyzed in the liver and hepatic lymph nodes (HLN) from sheep immunized with partially ... -
Expresión génica de IL4 e INFΓ en hígado y nódulo linfático hepático en ovejas vacunadas con CL1 infectadas con Fasciola hepática
Pacheco, I.L.; Abril, Nieves; Morales-Prieto, N.; Bautista Pérez, María José; Ruiz, M.Teresa; Zafra Leva, Rafael; Escamilla, Alejandro; Martínez-Moreno, Álvaro; Pérez, José (2017-11-30) -
Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep
Escamilla, Alejandro; Bautista, M.J.; Zafra Leva, Rafael; Pacheco, I.L.; Ruiz, M.Teresa; Martínez Cruz, Setefilla; Méndez, A.; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Elsevier, 2016)The aim of the present work was to evaluate the number of apoptotic eosinophils in the livers of sheep experimentally infected with Fasciola hepatica during the migratory and biliary stages of infection. Four groups (n = ... -
Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
Pacheco, Isabel; Abril, Nieves; Zafra Leva, Rafael; Molina-Hernández, Verónica; Morales‑Prieto2, Noelia; Bautista, M.J.; Ruiz-Campillo, María Teresa; Pérez Caballero, Raúl; Martínez Moreno, Álvaro; Pérez, José (Springer, 2018)The expression of T regulatory cells (Foxp3), regulatory (interleukin [IL]-10 and transforming growth factor beta [TGF-β]) and proinflammatory (tumor necrosis factor alpha [TNF-α] and interleukin [IL]-1β) cytokines was ... -
Fasciola hepatica primoinfections and reinfections in sheep drive distinct Th1/Th2/Treg immune responses in liver and hepatic lymph node at early and late stages
Ruiz-Campillo, María Teresa; Barrero‑Torres, Diana María; Abril, Nieves; Pérez, José; Zafra Leva, Rafael; Buffoni Perazzo, Leandro; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Molina-Hernández, Verónica (Springer, 2023)The expression of proinflammatory (IL-1β, IFN-γ, TNF-α) and regulatory (IL-10, TGF-β, IL-4) cytokines, as well as the transcription factor FoxP3, was quantified in the liver and hepatic lymph node (HLN) of sheep primoinfected ... -
Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
Flores Velazquez, Luis Miguel; Ruiz-Campillo, María Teresa; Herrera-Torres, Guillem; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Zafra Leva, Rafael; Buffoni Perazzo, Leandro; Rufino-Moya, Pablo José; Molina-Hernández, Verónica; Pérez, José (Frontiers, 2023)Fasciola hepatica has a worldwide distribution causing important economic losses in animal production industry. Human fasciolosis is considered an emerging zoonosis in areas of Andean America, Asia, and Africa. The control ... -
Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach
Buffoni Perazzo, Leandro; Garza-Cuartero, Laura; Pérez Caballero, Raúl; Zafra Leva, Rafael; Martínez-Moreno, Francisco Javier; Molina-Hernández, Verónica; Pérez, José; Martínez Moreno, Álvaro; Mulcahy, Grace (Springer, 2020)Background: Fasciolosis is one of the most important parasitic diseases of livestock. The need for better control strategies gave rise to the identification of various vaccine candidates. The recombinant form of a member ... -
Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica
Pacheco, I.L.; Abril, Nieves; Zafra Leva, Rafael; Morales-Prieto, N.; Molina Hernández, Verónica; Ruiz, M.T.; Pérez Caballero, Raúl; Martínez-Moreno, Álvaro; Pérez, José (Nature.com, 2019)The aim of this study was to validate reference genes for gene normalisation using qRT-PCR in hepatic lymph nodes (HLN) and livers from sheep infected with Fasciola hepatica during early and late stages of infection. To ... -
Revealing the Prevalence of Toxoplasma in Sierra Morena’s Wild Boar: An ELISA-Based Study Using Meat Juice
Castillo-Castillo, José María; Rufino-Moya, Pablo José; Martínez Moreno, Álvaro; Salvador Castaño, Ángela; Martínez-Moreno, Francisco Javier; Zafra Leva, Rafael (MDPI, 2024)This research work focused on the prevalence of Toxoplasma gondii in wild boar from the Sierra Morena region. We conducted an ELISA analysis using meat juice samples. A total of 892 samples from six hunting seasons (2013–2019) ... -
The Sigma Class Glutathione Transferase from the Liver Fluke Fasciola hepatica
LaCourse, E. James; Perally, Samirah; Morphew, Russell M.; Moxon, Joseph V.; Prescott, Mark; Dowling, David J.; O'Neill, Sandra M.; Kipar, Anja; Hetzel, Udo; Hoey, Elizabeth; Brophy, Peter M.; Pérez, José; Zafra Leva, Rafael; Buffoni Perazzo, Leandro (Public Library Of Science (PLOS), 2012)Background: Liver fluke infection of livestock causes economic losses of over US$ 3 billion worldwide per annum. The disease is increasing in livestock worldwide and is a re-emerging human disease. There are currently no ...