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dc.contributor.authorManso Cobos, Isabel
dc.contributor.authorIbáñez García, María Isabel
dc.contributor.authorPeña Moreno, Fernando de la
dc.contributor.authorSáez Melero, Lara Paloma
dc.contributor.authorLuque-Almagro, Víctor Manuel
dc.contributor.authorCastillo Rodríguez, Francisco
dc.contributor.authorRoldán Ruiz, María Dolores
dc.contributor.authorPrieto Jiménez, María Auxiliadora
dc.contributor.authorMoreno-Vivián, C.
dc.date.accessioned2017-12-07T11:59:56Z
dc.date.available2017-12-07T11:59:56Z
dc.date.issued2015
dc.identifier.citationMicrobial Cell Factories 14:77 (2015)es_ES
dc.identifier.urihttp://hdl.handle.net/10396/15667
dc.description.abstractBackground: Cyanide is one of the most toxic chemicals produced by anthropogenic activities like mining and jewelry industries, which generate wastewater residues with high concentrations of this compound. Pseudomonas pseudoalcaligenes CECT5344 is a model microorganism to be used in detoxification of industrial wastewaters containing not only free cyanide (CN−) but also cyano-derivatives, such as cyanate, nitriles and metal-cyanide complexes. Previous in silico analyses suggested the existence of genes putatively involved in metabolism of short chain length (scl-) and medium chain length (mcl-) polyhydroxyalkanoates (PHAs) located in three different clusters in the genome of this bacterium. PHAs are polyesters considered as an alternative of petroleum-based plastics. Strategies to optimize the bioremediation process in terms of reducing the cost of the production medium are required. Results: In this work, a biological treatment of the jewelry industry cyanide-rich wastewater coupled to PHAs production as by-product has been considered. The functionality of the pha genes from P. pseudoalcaligenes CECT5344 has been demonstrated. Mutant strains defective in each proposed PHA synthases coding genes (Mpha−, deleted in putative mcl-PHA synthases; Spha−, deleted in the putative scl-PHA synthase) were generated. The accumulation and monomer composition of scl- or mcl-PHAs in wild type and mutant strains were confirmed by gas chromatographymass spectrometry (GC–MS). The production of PHAs as by-product while degrading cyanide from the jewelry industry wastewater was analyzed in batch reactor in each strain. The wild type and the mutant strains grew at similar rates when using octanoate as the carbon source and cyanide as the sole nitrogen source. When cyanide was depleted from the medium, both scl-PHAs and mcl-PHAs were detected in the wild-type strain, whereas scl-PHAs or mcl-PHAs were accumulated in Mpha− and Spha−, respectively. The scl-PHAs were identified as homopolymers of 3-hydroxybutyrate and the mcl-PHAs were composed of 3-hydroxyoctanoate and 3-hydroxyhexanoate monomers. Conclusions: These results demonstrated, as proof of concept, that talented strains such as P. pseudoalcaligenes might be applied in bioremediation of industrial residues containing cyanide, while concomitantly generate by-products like polyhydroxyalkanoates. A customized optimization of the target bioremediation process is required to gain benefits of this type of approaches.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBioMed Centrales_ES
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subjectAlkalophilees_ES
dc.subjectBioplasticses_ES
dc.subjectCyanide degradationes_ES
dc.subjectPolyhydroxyalkanoateses_ES
dc.subjectPseudomonases_ES
dc.titlePseudomonas pseudoalcaligenes CECT5344, a cyanide‑degrading bacterium with by‑product (polyhydroxyalkanoates) formation capacityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1186/s12934-015-0267-8es_ES
dc.relation.projectIDGobierno de España. PET2008_0048es_ES
dc.relation.projectIDGobierno de España. BIO2011-30026-C02-02es_ES
dc.relation.projectIDGobierno de España. BIO2013-44878-Res_ES
dc.relation.projectIDJunta de Andalucía. CVI-7560es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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