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dc.contributor.authorGordon, Ana
dc.contributor.authorGarrido-Gracia, José C.
dc.contributor.authorMillán, Yolanda
dc.contributor.authorMartín de las Mulas González-Albo, Juana
dc.contributor.authorSánchez Criado, J.E.
dc.contributor.authorAguilar, Rafaela
dc.contributor.authorGuil-Luna, Silvia
dc.date.accessioned2017-01-18T11:13:32Z
dc.date.available2017-01-18T11:13:32Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/10396/14325
dc.description.abstractAdministration of human FSH(hFSH) to cyclic rats during the dioestrous phase attenuates progesterone receptor (PR)-dependent events of the preovulatory LH surge in pro-oestrus. The increased bioactivity of the putative ovarian gonadotropin surge inhibiting/attenuating factor induced by hFSH treatment is not associated with a decrease in PR protein expression, and the possibility of its association at a PR posttranslational effect has been raised. The present experiments aimed to analyse PR phosphorylation status in the gonadotrope of ratswith impaired LH secretion induced by in vivo hFSH injection. Two experimental approaches were used. First, incubated pro-oestrous pituitaries from hFSH-injected cycling and oestrogen-treated ovariectomized (OVX) rats were used to analyze the effect of calyculin, an inhibitor of intracellular phosphatases, on PR-dependent LH release, which was measured in the incubation medium by RIA. Second, pituitaries taken from hFSH-injected intact cycling and OVX rats and later incubated with P or GNRH1 were used to assess the phosphorylation rate of gonadotrope. The latter was analysed in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry using a MAB that recognizes the phosphorylated (p) form of PR at Ser294. Calyculin reduced the ovary-mediated inhibition of hFSH in GNRH1-stimulated LH secretion. In addition, the immunohistochemical expression of pSer294 PR was significantly reduced after ovarian stimulation with hFSH in pituitaries frompro-oestrous rats incubated with PorGNRH1.Altogether, these results suggested that the ovarian-dependent inhibitory effect of FSH injection on the preovulatory LH secretion in the rat may involve an increase in dephosphorylation of PR.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherSociety for Reproduction and Fertilityes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceReproduction 137, 151-159 (2009)es_ES
dc.subjectEstimulación ováricaes_ES
dc.titleOvarian stimulation with FSH reduces phosphorylation of gonadotrope progesterone receptor and LH secretion in the rates_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1530/REP-08-0318es_ES
dc.relation.projectIDGobierno de España. BFU2005-01443es_ES
dc.relation.projectIDGobierno de España. AGL2006-09016/GANes_ES
dc.relation.projectIDGobierno de España. BFU2008-00480es_ES
dc.relation.projectIDJunta de Andalucía. P07-CVI-2559es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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