Mostrar el registro sencillo del ítem
TGF-β Prevents Phosphate-Induced Osteogenesis through Inhibition of BMP and Wnt/β-Catenin Pathways
dc.contributor.author | Guerrero Pavón, Fátima | |
dc.contributor.author | Herencia, Carmen | |
dc.contributor.author | Almadén Peña, Yolanda | |
dc.contributor.author | Martínez-Moreno, Julio M. | |
dc.contributor.author | Montes de Oca, Addy | |
dc.contributor.author | Rodríguez-Ortiz, Maria E. | |
dc.contributor.author | Díaz Tocados, Juan Manuel | |
dc.contributor.author | Florio, Mónica | |
dc.contributor.author | López, Ignacio | |
dc.contributor.author | Richards, William G. | |
dc.contributor.author | Rodríguez Portillo, Mariano | |
dc.contributor.author | Aguilera Tejero, Escolástico | |
dc.contributor.author | Muñoz-Castañeda, Juan R. | |
dc.date.accessioned | 2017-11-23T10:53:00Z | |
dc.date.available | 2017-11-23T10:53:00Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | http://hdl.handle.net/10396/15501 | |
dc.description.abstract | Background: Transforming growth factor-b (TGF-b) is a key cytokine during differentiation of mesenchymal stem cells (MSC) into vascular smooth muscle cells (VSMC). High phosphate induces a phenotypic transformation of vascular smooth muscle cells (VSMC) into osteogenic-like cells. This study was aimed to evaluate signaling pathways involved during VSMC differentiation of MSC in presence or not of high phosphate. Results: Our results showed that TGF-b induced nuclear translocation of Smad3 as well as the expression of vascular smooth muscle markers, such as smooth muscle alpha actin, SM22a, myocardin, and smooth muscle-myosin heavy chain. The addition of high phosphate to MSC promoted nuclear translocation of Smad1/5/8 and the activation of canonical Wnt/bcatenin in addition to an increase in BMP-2 expression, calcium deposition and alkaline phosphatase activity. The administration of TGF-b to MSC treated with high phosphate abolished all these effects by inhibiting canonical Wnt, BMP and TGF-b pathways. A similar outcome was observed in high phosphate-treated cells after the inhibition of canonical Wnt signaling with Dkk-1. Conversely, addition of both Wnt/b-catenin activators CHIR98014 and lithium chloride enhanced the effect of high phosphate on BMP-2, calcium deposition and alkaline phosphatase activity. Conclusions: Full VSMC differentiation induced by TGF-b may not be achieved when extracellular phosphate levels are high. Moreover, TGF-b prevents high phosphate-induced osteogenesis by decreasing the nuclear translocation of Smad 1/5/8 and avoiding the activation of Wnt/b-catenin pathway. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.source | PLoS ONE 9(6): e101910 (2014) | es_ES |
dc.subject | Vascular calcification | es_ES |
dc.subject | Osteogenesis | es_ES |
dc.subject | Growth factor-β | es_ES |
dc.subject | Phosphates | es_ES |
dc.subject | Mesenchymal stem cells | es_ES |
dc.subject | Cell differentiation | es_ES |
dc.subject | Phosphatases | es_ES |
dc.subject | DAPI staining | es_ES |
dc.title | TGF-β Prevents Phosphate-Induced Osteogenesis through Inhibition of BMP and Wnt/β-Catenin Pathways | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0089179 | es_ES |
dc.relation.projectID | Junta de Andalucía. P09-CTS-5205 | es_ES |
dc.relation.projectID | Junta de Andalucía. PI-0127 | es_ES |
dc.relation.projectID | Instituto de Salud Carlos III. FIS PI11/02055 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |