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dc.contributor.authorPérez Jiménez, Margarita
dc.contributor.authorBesnard, Guillaume
dc.contributor.authorDorado, G.
dc.contributor.authorHernández, Pilar
dc.date.accessioned2017-12-01T09:55:00Z
dc.date.available2017-12-01T09:55:00Z
dc.date.issued2013
dc.identifier.urihttp://hdl.handle.net/10396/15623
dc.description.abstractOlive oil traceability remains a challenge nowadays. DNA analysis is the preferred approach to an effective varietal identification, without any environmental influence. Specifically, olive organelle genomics is the most promising approach for setting up a suitable set of markers as they would not interfere with the pollinator variety DNA traces. Unfortunately, plastid DNA (cpDNA) variation of the cultivated olive has been reported to be low. This feature could be a limitation for the use of cpDNA polymorphisms in forensic analyses or oil traceability, but rare cpDNA haplotypes may be useful as they can help to efficiently discriminate some varieties. Recently, the sequencing of olive plastid genomes has allowed the generation of novel markers. In this study, the performance of cpDNA markers on olive oil matrices, and their applicability on commercial Protected Designation of Origin (PDO) oils were assessed. By using a combination of nine plastid loci (including multi-state microsatellites and short indels), it is possible to fingerprint six haplotypes (in 17 Spanish olive varieties), which can discriminate high-value commercialized cultivars with PDO. In particular, a rare haplotype was detected in genotypes used to produce a regional high-value commercial oil. We conclude that plastid haplotypes can help oil traceability in commercial PDO oils and set up an experimental methodology suitable for organelle polymorphism detection in the complex olive oil matrices.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourcePLoS ONE 8(8): e70507 (2013)es_ES
dc.subjectVegetable oilses_ES
dc.subjectOlive treeses_ES
dc.subjectGenetic locies_ES
dc.subjectHaplotypeses_ES
dc.subjectOilses_ES
dc.subjectPlastidses_ES
dc.subjectPolymerase chain reactiones_ES
dc.subjectOliveses_ES
dc.titleVarietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profilinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0070507es_ES
dc.relation.projectIDGobierno de España. AGL2010-17316es_ES
dc.relation.projectIDJunta de Andalucía. 041/C/2007es_ES
dc.relation.projectIDJunta de Andalucía. 75/C/2009es_ES
dc.relation.projectIDJunta de Andalucía. 56/C/2010es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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