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dc.contributor.authorCardoso-Toset, Fernando
dc.contributor.authorLuque, Inmaculada
dc.contributor.authorAmarilla, Shyrley P.
dc.contributor.authorFernández, Layla
dc.contributor.authorHuerta Lorenzo, Belén
dc.contributor.authorCarrasco Otero, Librado
dc.contributor.authorRuiz, Pilar
dc.contributor.authorGómez-Laguna, J.
dc.date.accessioned2017-12-22T12:20:27Z
dc.date.available2017-12-22T12:20:27Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10396/15786
dc.description.abstractFree-range pigs can be infected by Mycobacterium tuberculosis complex (MTC) and may contribute to the spread of bovine tuberculosis (bTB). In the present study, the diagnostic values of bacteriological culture, a duplex real-time quantitative PCR and an antibody ELISA were evaluated in an abattoir study of submandibular lymph nodes and serum samples from 73 pigs with and without lesions consistent with bTB. The duplex qPCR was an accurate method for diagnosis of TB in pigs (specificity 100%; sensitivity 80%). Combining qPCR with histopathology improved sensitivity and had very good concordance (κ = 0.94) with the reference method. Serological results suggest that the antibody ELISA can be used for monitoring herds but not individuals.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subjectTuberculosises_ES
dc.subjectPigses_ES
dc.subjectDiagnosises_ES
dc.subjectReal-time quantitative PCRes_ES
dc.subjectELISAes_ES
dc.titleEvaluation of rapid methods for diagnosis of tuberculosis in slaughtered free-range pigses_ES
dc.typeinfo:eu-repo/semantics/preprintes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.tvjl.2015.01.022es_ES
dc.relation.projectIDJunta de Andalucía. AGR-2685-2012es_ES
dc.relation.projectIDGobierno de España. IDI-20111633es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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