Rangel Zúñiga, Oriol AlbertoCamargo García, A.Marín, CarmenPeña Orihuela, JudhitPérez-Martínez, PabloDelgado-Lista, JavierGonzález Guardia, LorenaYubero-Serrano, Elena M.Tinahones, Francisco J.Malagón, María M.Pérez-Jiménez, FranciscoRoche, Helen M.López-Miranda, José2017-12-07T10:32:37Z2017-12-07T10:32:37Z2015http://hdl.handle.net/10396/15665Background: Metabolic syndrome is a multi-component disorder associated to a high risk of cardiovascular disease.
Its etiology is the result of a complex interaction between genetic and environmental factors, including dietary
habits. We aimed to identify the target proteins modulated by the long-term consumption of four diets differing in
the quality and quantity of lipids in the whole proteome of peripheral blood mononuclear cells (PBMC).
Results: A randomized, controlled trial conducted within the LIPGENE study assigned 24 MetS patients for 12 weeks
each to 1 of 4 diets: a) high-saturated fatty acid (HSFA), b) high-monounsaturated fatty acid (HMUFA), c) low-fat,
high-complex carbohydrate diets supplemented with placebo (LFHCC) and d) low-fat, high-complex carbohydrate
diets supplemented with long chain (LC) n-3 polyunsaturated fatty acids (PUFA) (LFHCC n-3). We analyzed the
changes induced in the proteome of both nuclear and cytoplasmic fractions of PBMC using 2-D proteomic analysis.
Sixty-seven proteins were differentially expressed after the long-term consumption of the four diets. The HSFA diet
induced the expression of proteins responding to oxidative stress, degradation of ubiquitinated proteins and DNA
repair. However, HMUFA, LFHCC and LFHCC n-3 diets down-regulated pro-inflammatory and oxidative stress-related
proteins and DNA repairing proteins.
Conclusion: The long-term consumption of HSFA, compared to HMUFA, LFHCC and LFHCC n-3, seems to increase the
cardiovascular disease (CVD) risk factors associated with metabolic syndrome, such as inflammation and oxidative stress,
and seem lead to DNA damage as a consequence of high oxidative stress.application/pdfengBioMed Centralhttps://creativecommons.org/licenses/by-nc-nd/4.0/BMC Genomics 16:509 (2015)Metabolic syndromeProteomicsInflammationOxidative stressDNA damageProteome from patients with metabolic syndrome is regulated by quantity and quality of dietary lipidsinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12864-015-1725-8Unión Europea. 505944 (LIPGENE)Gobierno de España. AGL2004-07907Gobierno de España. AGL2006-01979Gobierno de España. AGL2009-12270Instituto de Salud Carlos III. CB06/03/0047 (CIBEROBN)Junta de Andalucía. P06-CTS-01425Junta de Andalucía. CTS-03039Junta de Andalucía. 06/128Junta de Andalucía. 07/43Junta de Andalucía. PI-0193info:eu-repo/semantics/openAccess