Pharmacokinetic/pharmacodynamic modeling of benazepril and benazeprilat after administration of intravenous and oral doses of benazepril in healthy horses
Autor
Serrano-Rodríguez, Juan Manuel
Gómez Díez, María
Esgueva, María
Castejón Riber, Cristina
Mena Bravo, Antonio
Priego-Capote, Feliciano
Ayala-Soldado, Nahúm
Serrano-Caballero, J.M.
Muñoz, Ana
Editor
ElsevierFecha
2017Materia
Angiotensin-converting enzyme inhibitorsBenazeprilat
Benazepril
Pharmacokinetic-pharmacodynamic
Horses
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Pharmacokinetic and pharmacodynamic (PK/PD) properties of the angiotensin- converting enzyme inhibitor (ACEI) benazeprilat have not been evaluated in horses. This study was designed to establish PK profiles for benazepril and benazeprilat after intravenous (IV) and oral (PO) administration of benazepril using a PK/PD model. This study also aims to determine the effects of benazeprilat on serum angiotensin converting enzyme (ACE), selecting the most appropriate dose that suppresses ACE activity. Six healthy horses in a crossover design received IV benazepril at 0.50 mg/kg and PO at doses 0 (placebo), 0.25, 0.50 and 1.00 mg/kg. Blood pressures (BP) were measured and blood samples were obtained at different times in order to measure serum drug concentrations and serum ACE activity, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and spectrophotometry, respectively. Systemic bioavailability of benazeprilat after PO benazepril was 3-4%. Maximum ACE inhibitions from baseline were 99.63% (IV benazepril), 6.77% (placebo) and 78.91%, 85.74% and 89.51% (for the three PO benazepril doses). Significant differences in BP were not found. Although oral availability was low, benazeprilat 1.00 mg/kg, reached sufficient serum concentrations to induce long lasting serum ACE inhibitions (between 88 and 50%) for the first 48 h. Additional research on benazepril administration in equine patients is indicated.