Effect of Time, Temperature, and Inoculum Density on Reproduction of Pratylenchus thornei in Carrot Disk Cultures
Autor
Trapero-Casas, J.L.
Jiménez Díaz, Rafael M.
Castillo, Pablo
Editor
The Society of NematologistsFecha
1995Materia
CarrotInoculum density
Lesion nematode
Monoxenic culture
Nematode
Pratylenchus thornei
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Reproduction ofPratylenchus tho,'nei on carrot disk cultures at different time periods after
inoculation, temperature, and initial inoculum density was studied. At 25 C and with an initial
inoculum of 25 females per disk, the final nematode population increased with increasing time after
inoculation, although the populations after 25 and 50 days were not different. Nematode numbers
increased by 1,255-fold and 3,619-fold at 75 and 100 days, respectively. Over 35 days incubation at
15, 20, 25, and 30 C, the nematode multiplied 1.8, 8.4, 10.5, and 0.4 times, respectively. The
optimum temperature for reproduction was between 20 and 25 C, and the nematode life cycle was
completed in about 25-35 days. Increasing nematode inoculum (25, 50, 100, 500, 1,000 nematodes
per disk) increased the final nematode population but did not increase reproduction rate, the highest
being 25.3 at an initial inoculum density of 100 nematodes per disk.