Show simple item record

dc.contributor.authorGelis, Samuel
dc.contributor.authorCurto Rubio, Miguel
dc.contributor.authorValledor, Luis
dc.contributor.authorGonzález, Asier
dc.contributor.authorAriño, Joaquín
dc.contributor.authorJorrín-Novo, Jesús V.
dc.contributor.authorRamos, José
dc.date.accessioned2013-07-02T12:11:15Z
dc.date.available2013-07-02T12:11:15Z
dc.date.issued2012
dc.identifier.urihttp://hdl.handle.net/10396/10654
dc.description.abstractSaccharomyces cerevisiaewild-type (BY4741) and the correspondingmutant lacking the plasma membrane main potassium uptake systems (trk1,trk2) were used to analyze the consequences of K+ starvation following a proteomic approach. In order to trigger high-affinity mode of potassium transport, cells were transferred to potassium-freemedium.Protein profilewas followed by two-dimensional (2-D) gels in samples taken at 0, 30, 60, 120, 180, and 300 min during starvation.We observed a general decrease of protein content during starvation that was especially drastic in the mutant strain as it was the case of an important number of proteins involved in glycolysis. On the contrary, we identified proteins related to stress response and alternative energetic metabolism that remained clearly present. Neural networkbased analysis indicated that wild type was able to adapt much faster than the mutant to the stress process. We conclude that complete potassium starvation is a stressful process for yeast cells, especially for potassium transport mutants, and we propose that less stressing conditions should be used in order to study potassium homeostasis in yeast.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBlackwell Publishing Ltdes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceMicrobiologyOpen 1 (2), 182-193 (2012)es_ES
dc.subject2D-gelses_ES
dc.subjectPotassium homeostasises_ES
dc.subjectSacharomyces cerevisaees_ES
dc.subjectTRK1es_ES
dc.subjectTRK2es_ES
dc.titleAdaptation to potassium starvation of wild-type and K+-transport mutant (trk1,2) of Saccharomyces cerevisiae: 2-dimensional gel electrophoresis-based proteomic approaches_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record