• Flow-cytometric analysis of membrane integrity of stallion sperm in the face of agglutination: the “zombie sperm” dilemma 

  Ortiz, Isabel; Felix, Matheus; Resende, Helene; Ramirez-Agamez, Luisa; Love, Charles C; Hinrichs, Katrin (Springer, 2021)

  Purpose To define the effect of sperm agglutination, associated with incubation under capacitating conditions, on accuracy of membrane assessment via flow cytometry and to develop methods to mitigate that effect. Met ...

• Comparison of different mathematical models to assess seasonal variations in the longevity of DNA integrity of cooled-stored stallion sperm 

  Ortiz, Isabel; Quiñones-Perez, Carlota; Hidalgo, Manuel; Consuegra, Cesar; Diaz-Jimenez, Maria; Dorado, Jesus; Vega-Pla, Jose Luis; Crespo, Francisco (Wiley, 2020)

  Dynamic assessment of sperm DNA fragmentation (SDF) has shown to give fuller understanding of stallion semen quality; however, there have been limited attempts to use this parameter to investigate seasonal changes in ...

• Comparison of DNA fragmentation of frozen-thawed epididymal sperm of dogs using Sperm Chromatin Structure Analysis and Sperm Chromatin Dispersion test 

  Ortiz, Isabel; Urbano, Maite; Dorado, Jesus; Morrell, Jane; Al-Essawe, E; Johanisson, A; Hidalgo, Manuel (Elsevier, 2017)

  The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis ...

• New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models 

  Ortiz, Isabel; Dorado, Jesus; Morrell, Jane M.; Gosalvez, Jaime; Crespo, Francisco; Jimenez, Juan M.; Hidalgo, Manuel (2017)

  Background Sperm DNA fragmentation (sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select ...

• Vitrification induces critical subcellular damages in ram spermatozoa 

  Arando Arbulu, Ander; Delgado-Bermejo, J.V.; Arrebola, F.; León Jurado, J.M.; Alcalá, Carlos J.; Pérez-Marín, C.C. (Elsevier, 2019)

  The aim of the present study was to analyse morphological variations in ovine spermatozoa subjected to different cryopreservation protocols using high resolution imaging techniques. Ejaculates were pooled and diluted in ...

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