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Preliminary Evaluation of a Multiplex Reverse Transcription-PCR Assay Combined with a New DNA Chip Hybridization Assay for Detecting Respiratory Syncytial Virus
dc.contributor.author | Causse, Manuel | |
dc.contributor.author | García-Mayorgas, Ángel D. | |
dc.contributor.author | Gutiérrez-Aroca, Juan-Bautista | |
dc.contributor.author | Casal Román, Manuel | |
dc.date.accessioned | 2018-12-19T09:50:00Z | |
dc.date.available | 2018-12-19T09:50:00Z | |
dc.date.issued | 2007 | |
dc.identifier.uri | http://hdl.handle.net/10396/17595 | |
dc.description.abstract | DNA chips represent a major advance in microbiology laboratories, enabling the detection of a wide range of possible pathogens using a single test. This study compared a multiplex reverse transcription-PCR combined with DNA chip hybridization (ProDect BCS RV chip; bcs Biotech) with the indirect immunofluorescence test commonly used to detect respiratory viruses. A total of 39 respiratory viruses (38 respiratory syncytial viruses [RSVs] and 1 influenza A virus) were detected in samples from 96 patients using the immunofluorescence test, while 36 viruses (34 RSV, 1 influenza A virus, and 1 influenza B virus) were detected by the DNA chip technique. Results showed a good level of agreement between the two tests for RSV detection; the incidence of other viruses was low, since samples were taken from patients with suspected bronchiolitis. DNA chips displayed high sensitivity (94.6%) and specificity (100%). | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Microbilogy | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Journal of Clinical Microbiology 45 (11), 3811-3813 (2007) | es_ES |
dc.subject | DNA chips | es_ES |
dc.subject | RSV detection | es_ES |
dc.title | Preliminary Evaluation of a Multiplex Reverse Transcription-PCR Assay Combined with a New DNA Chip Hybridization Assay for Detecting Respiratory Syncytial Virus | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.1128/JCM.00345-07 | es_ES |
dc.relation.projectID | Gobierno de España. REIPI RD06/0008 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |