Evaluation of the GenoType Mycobacteria Direct Assay for Detection of Mycobacterium tuberculosis Complex and Four Atypical Mycobacterial Species in Clinical Samples
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Autor
Franco Alvárez de Luna, Francisco
Ruiz, Pilar
Gutiérrez-Aroca, Juan-Bautista
Casal Román, Manuel
Editor
American Society for MicrobilogyFecha
2006Materia
GTMDM. tuberculosis
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We evaluated GenoType Mycobacteria Direct (GTMD), a novel commercial assay based on nucleic acid sequence-based amplification technology, for the detection of Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, and M. malmoense directly from clinical specimens. A total of 134 respiratory and extrapulmonary samples from 65 patients were processed. Sensitivity, specificity, positive predictive, and negative predictive values for GTMD were 92, 100, 100, and 77%, respectively. The GMTD technique is useful, reliable, and rapid when used during the normal routine of a clinical laboratory.