Listar Producción Científica por autor "dfbb9bc4-800d-436e-ada4-0922ffc1a15e"
Mostrando ítems 1-20 de 23
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A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep
Molina Hernández, Verónica; Ruiz-Campillo, María Teresa; Martínez-Moreno, Francisco Javier; Buffoni Perazzo, Leandro; Martínez-Moreno, Álvaro; Zafra Leva, Rafael; Escamilla, Alejandro; Pérez Caballero, Raúl; Pérez Arévalo, José (MDPI, 2021)Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the ... -
Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep
Escamilla, Alejandro; Pérez Caballero, Raúl; Zafra Leva, Rafael; Bautista, M.J.; Pacheco, I.L.; Ruiz, M.Teresa; Martínez Cruz, Setefilla; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Elsevier, 2017)Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present ... -
Cambios celulares y moleculares en el perioteneo asociados con la patología hepática en fases tempranas de la infección con fasciola hepática en ovejas
Molina Hernández, Verónica; Ruiz-Campillo, María Teresa; Escamilla, Alejandro; Pérez, José; Martínez-Moreno, Álvaro; Cwiklinski, Krystyna; Stevenson, Michael; Donnelly, Sheila; Dalton, John P. (Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas, 2016) -
Caracterización de macrófagos peritoneales de ovejas en fases tempranas de infección experimental con fasciola hepática
Zafra Leva, Rafael; Pacheco, I.L.; Escamilla, Alejandro; Caballero, R.; Buffoni Perazzo, Leandro; Molina Hernández, Verónica; Martínez-Moreno, Álvaro; Pérez, José; Ruiz-Campillo, María Teresa (Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas, 2016) -
Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica
Ruiz-Campillo, María Teresa; Molina-Hernández, Verónica; Bautista, María José; Pacheco, I.L.; Zafra, Rafael; Buffoni Perazzo, Leandro; Martínez-Moreno, Francisco Javier; Martínez Moreno, Álvaro; Pérez, José (Springer, 2020)Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment ... -
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis
Pérez Caballero, Raúl; Martínez-Moreno, Francisco Javier; Zafra, Rafael; Molina-Hernández, Verónica; Pacheco, I.L.; Ruiz-Campillo, María Teresa; Escamilla, Alejandro; Pérez, José; Martínez Moreno, Álvaro; Buffoni Perazzo, Leandro (Springer, 2018)Background: The peritoneal cell populations (PCP) are thought to play a crucial role during the early immune response in Fasciola hepatica infection while newly excysted juveniles (NEJ) are migrating in the peritoneal ... -
Distribución de linfocitos FOXP3 reguladores en hígado y nódulos linfáticos de ovejas y cabras infectadas con fasciola hepática
Escamilla, Alejandro; Zafra Leva, Rafael; Bautista Pérez, María José; Pacheco, I.L.; Ruiz-Campillo, María Teresa; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Universidad de Córdoba. Departamento de Anatomía y Anatomía Patológica Comparadas, 2016) -
Distribution of Foxp3+ T cells in the liver and hepatic lymph nodes of goats and sheep experimentally infected with Fasciola hepatica
Escamilla, Alejandro; Zafra Leva, Rafael; Pérez, José; McNeilly, T.N.; Pacheco, I.L.; Buffoni Perazzo, Leandro; Martínez-Moreno, Francisco Javier; Molina Hernández, Verónica; Martínez-Moreno, Álvaro (Elsevier, 2016)Foxp3 regulatory T cells (Tregs) are now considered to play a key role in modulation of immune responses during parasitic helminth infections. Immunomodulation is a key factor in Fasciola hepatica infection; however, the ... -
Efficacy of a multivalent vaccine against Fasciola hepatica infection in sheep
Zafra, Rafael; Buffoni Perazzo, Leandro; Pérez Caballero, Raúl; Molina-Hernández, Verónica; Ruiz-Campillo, María Teresa; Pérez, José; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier (Springer, 2021)In this work we report the protection found in a vaccination trial performed in sheep with two different vaccines composed each one by a cocktail of antigens (rCL1, rPrx, rHDM and rLAP) formulated in two different adjuvants ... -
Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica infection
Pérez Caballero, Raúl; Buffoni Perazzo, Leandro; Martínez-Moreno, Francisco Javier; Zafra, Rafael; Molina-Hernández, Verónica; Pérez, José; Martínez Moreno, Álvaro (Springer, 2018)Background: The majority of vaccination studies against infection with F. hepatica in a natural host have been conducted at the late stage of the infection when the host's immune response is already immunomodulated by the ... -
Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep
Escamilla, Alejandro; Bautista, M.J.; Zafra Leva, Rafael; Pacheco, I.L.; Ruiz, M.Teresa; Martínez Cruz, Setefilla; Méndez, A.; Martínez-Moreno, Álvaro; Molina Hernández, Verónica; Pérez, José (Elsevier, 2016)The aim of the present work was to evaluate the number of apoptotic eosinophils in the livers of sheep experimentally infected with Fasciola hepatica during the migratory and biliary stages of infection. Four groups (n = ... -
Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection
Pacheco, Isabel; Abril, Nieves; Zafra, Rafael; Molina-Hernández, Verónica; Morales‑Prieto2, Noelia; Bautista, M.J.; Ruiz-Campillo, María Teresa; Pérez Caballero, Raúl; Martínez Moreno, Álvaro; Pérez, José (Springer, 2018)The expression of T regulatory cells (Foxp3), regulatory (interleukin [IL]-10 and transforming growth factor beta [TGF-β]) and proinflammatory (tumor necrosis factor alpha [TNF-α] and interleukin [IL]-1β) cytokines was ... -
Fasciola hepatica primoinfections and reinfections in sheep drive distinct Th1/Th2/Treg immune responses in liver and hepatic lymph node at early and late stages
Ruiz-Campillo, María Teresa; Barrero‑Torres, Diana María; Abril, Nieves; Pérez, José; Zafra Leva, Rafael; Buffoni Perazzo, Leandro; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Molina-Hernández, Verónica (Springer, 2023)The expression of proinflammatory (IL-1β, IFN-γ, TNF-α) and regulatory (IL-10, TGF-β, IL-4) cytokines, as well as the transcription factor FoxP3, was quantified in the liver and hepatic lymph node (HLN) of sheep primoinfected ... -
Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development
Flores Velazquez, Luis Miguel; Ruiz-Campillo, María Teresa; Herrera-Torres, Guillem; Martínez Moreno, Álvaro; Martínez-Moreno, Francisco Javier; Zafra, Rafael; Buffoni Perazzo, Leandro; Rufino-Moya, Pablo José; Molina-Hernández, Verónica; Pérez, José (Frontiers, 2023)Fasciola hepatica has a worldwide distribution causing important economic losses in animal production industry. Human fasciolosis is considered an emerging zoonosis in areas of Andean America, Asia, and Africa. The control ... -
Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach
Buffoni Perazzo, Leandro; Garza-Cuartero, Laura; Pérez Caballero, Raúl; Zafra, Rafael; Martínez-Moreno, Francisco Javier; Molina-Hernández, Verónica; Pérez, José; Martínez Moreno, Álvaro; Mulcahy, Grace (Springer, 2020)Background: Fasciolosis is one of the most important parasitic diseases of livestock. The need for better control strategies gave rise to the identification of various vaccine candidates. The recombinant form of a member ... -
Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica
Pacheco, I.L.; Abril, Nieves; Zafra Leva, Rafael; Morales-Prieto, N.; Molina Hernández, Verónica; Ruiz, M.T.; Pérez Caballero, Raúl; Martínez-Moreno, Álvaro; Pérez, José (Nature.com, 2019)The aim of this study was to validate reference genes for gene normalisation using qRT-PCR in hepatic lymph nodes (HLN) and livers from sheep infected with Fasciola hepatica during early and late stages of infection. To ... -
Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica
Ruiz-Campillo, María Teresa; Molina Hernández, Verónica; Escamilla, Alejandro; Stevenson, Michael; Pérez, José; Martínez-Moreno, Álvaro; Donnelly, Sheila; Dalton, John P.; Cwiklinski, Krystyna (Nature, 2017)Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. ... -
Immunohistochemical analysis of knee chondral defect repair after autologous particulated cartilage and platelet-rich plasma treatment in sheep
Alcaide-Ruggiero, Lourdes; Molina-Hernández, Verónica; Morgaz, Juan; Fernández-Sarmiento, J. Andrés; Granados, María M.; Navarrete-Calvo, Rocío; Pérez, José; Quirós-Carmona, Setefilla; Carrillo, José M.; Cugat, Ramón; Domínguez, Juan M. (MDPI, 2023)This study performs an analysis that will enable the evaluation of the quality, durability, and structure of repaired cartilaginous extracellular matrix tissue using an autologous-based particulated autograft cartilage ... -
Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects
Alcaide-Ruggiero, Lourdes; Molina Hernández, Verónica; Granados Machuca, Mª del Mar; Domínguez Pérez, Juan Manuel (MDPI, 2021)Several collagen subtypes have been identified in hyaline articular cartilage. The main and most abundant collagens are type II, IX and XI collagens. The minor and less abundant collagens are type III, IV, V, VI, X, XII, ... -
Study of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model
Becerro Recio, David; Serrat, Judit; López García, Marta; Molina-Hernández, Verónica; Pérez Arévalo, José; Martínez Moreno, Álvaro; Sotillo, Javier; González-Miguel, Javier; Siles Lucas, Mar (Public Library Of Science (PLOS), 2022)Fasciola hepatica is a trematode parasite that infects animals and humans causing fasciolosis, a worldwide-distributed disease responsible for important economic losses and health problems. This disease is of growing public ...