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dc.contributor.authorJiménez-Marín, Ángeles
dc.contributor.authorCollado-Romero, Melania
dc.contributor.authorRamírez-Boo, M.
dc.contributor.authorArce Jiménez, Cristina
dc.contributor.authorGarrido, Juan J.
dc.date.accessioned2013-10-16T12:16:41Z
dc.date.available2013-10-16T12:16:41Z
dc.date.issued2009
dc.identifier.urihttp://hdl.handle.net/10396/11133
dc.description.abstractBackground: Once a list of differentially expressed genes has been identified from a microarray experiment, a subsequent post-analysis task is required in order to find the main biological processes associated to the experimental system. This paper describes two pathways analysis tools, ArrayUnlock and Ingenuity Pathways Analysis (IPA) to deal with the post-analyses of microarray data, in the context of the EADGENE and SABRE post-analysis workshop. Dataset employed in this study proceeded from an experimental chicken infection performed to study the host reactions after a homologous or heterologous secondary challenge with two species of Eimeria. Results: Analysis of the same microarray data source employing both commercial pathway analysis tools in parallel let to identify several biological and/or molecular functions altered in the chicken Eimeria maxima infection model, including several immune system related pathways. Biological functions differentially altered in the homologous and heterologous second infection were identified. Similarly, the effect of the timing in a homologous second infection was characterized by several biological functions. Conclusion: Functional analysis with ArrayUnlock and IPA provided information related to functional differences with the three comparisons of the chicken infection leading to similar conclusions. ArrayUnlock let an improvement of the annotations of the chicken genome adding InterPro annotations to the data set file. IPA provides two powerful tools to understand the pathway analysis results: the networks and canonical pathways that showed several pathways related to an adaptative immune responsees_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBioMed Centrales_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceBMC Proceedings 3 (Suppl 4) (2009)es_ES
dc.subjectPathways analysises_ES
dc.subjectExpressed geneses_ES
dc.titleBiological pathway analysis by ArrayUnlock and Ingenuity Pathway Analysises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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