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dc.contributor.authorSimova-Stoilova, Lyudmila P.
dc.contributor.authorRomero-Rodríguez, M. Cristina
dc.contributor.authorSánchez-Lucas, Rosa
dc.contributor.authorNavarro Cerrillo, Rafael M.
dc.contributor.authorMedina-Aunon, J. Alberto
dc.contributor.authorJorrín-Novo, Jesús V.
dc.date.accessioned2017-11-16T10:08:58Z
dc.date.available2017-11-16T10:08:58Z
dc.date.issued2015
dc.identifier.urihttp://hdl.handle.net/10396/15453
dc.description.abstractHolm oak is a dominant tree in the western Mediterranean region. Despite being well adapted to dry hot climate, drought is the main cause of mortality post-transplanting in reforestation programs. An active response to drought is critical for tree establishment and survival. Applying a gel-based proteomic approach, dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Stress was monitored by changes in water status, plant growth, and electrolyte leakage. Contrary to leaves, holm oak roots responded readily to water shortage at physiological level by growth inhibition, changes in water status and membrane stability. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and separated by two-dimensional electrophoresis. Coomassie colloidal stained gel images were analyzed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Data are available via ProteomeXchange with identifier PXD002484. Identified proteins were classified into functional groups: metabolism, protein biosynthesis and proteolysis, defense against biotic stress, cellular protection against abiotic stress, intracellular transport. Several enzymes of the carbohydrate metabolism decreased in abundance in roots under drought stress while some related to ATP synthesis and secondary metabolism increased. Results point at active metabolic adjustment and mobilization of the defense system in roots to actively counteract drought stress.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceFrontiers in Plant Science, 6: 627 (2015)es_ES
dc.subjectHolm oakes_ES
dc.subjectRootses_ES
dc.subjectDroughtes_ES
dc.subjectRecoveryes_ES
dc.subjectProteomicses_ES
dc.title2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortagees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fpls.2015.00627es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/271714 (QUIPROD)es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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