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dc.contributor.authorHerrera Uribe, Juber
dc.contributor.authorCollado-Romero, Melania
dc.contributor.authorZaldívar-López, Sara
dc.contributor.authorArce Jiménez, Cristina
dc.contributor.authorBautista, Rocío
dc.contributor.authorCarvajal, Ana
dc.contributor.authorCirera, Susanna
dc.contributor.authorGonzalo Claros, M.
dc.contributor.authorGarrido, Juan J.
dc.date.accessioned2018-10-17T10:27:53Z
dc.date.available2018-10-17T10:27:53Z
dc.date.issued2016
dc.identifier.urihttp://hdl.handle.net/10396/17309
dc.description.abstractInfected pork meat is an important source of non-typhoidal human salmonellosis. Understanding of molecular mechanisms involved in disease pathogenesis is important for the development of therapeutic and preventive strategies. Thus, hereby we study the transcriptional profiles along the porcine intestine during infection with Salmonella Typhimurium, as well as post-transcriptional gene modulation by microRNAs (miRNA). Sixteen piglets were orally challenged with S. Typhimurium. Samples from jejunum, ileum and colon, collected 1, 2 and 6 days post infection (dpi) were hybridized to mRNA and miRNA expression microarrays and analyzed. Jejunum showed a reduced transcriptional response indicating mild inflammation only at 2 dpi. In ileum inflammatory genes were overexpressed (e.g., IL-1B, IL-6, IL-8, IL1RAP, TNFα), indicating a strong immune response at all times of infection. Infection also down-regulated genes of the FXR pathway (e.g., NR1H4, FABP6, APOA1, SLC10A2), indicating disruption of the bile acid absorption in ileum. This result was confirmed by decreased high-density lipoprotein cholesterol in serum of infected pigs. Ileal inflammatory gene expression changes peaked at 2 dpi and tended to resolve at 6 dpi. Furthermore, miRNA analysis of ileum at 2 dpi revealed 62 miRNAs potentially regulating target genes involved in this inflammatory process (e.g., miR-374 and miR-451). In colon, genes involved in epithelial adherence, proliferation and cellular reorganization were down-regulated at 2 and 6 dpi. In summary, here we show the transcriptional changes occurring at the intestine at different time points of the infection, which are mainly related to inflammation and disruption of the bile acid metabolism. © 2016 Uribe et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherBiomed Centrales_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceVeterinary Research 47:11 (2016)es_ES
dc.subjectSalmonellosises_ES
dc.subjectInfected pork meates_ES
dc.titleTranscriptional analysis of porcine intestinal mucosa infected with Salmonella Typhimurium revealed a massive inflammatory response and disruption of bile acid absorption in ileumes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1186/s13567-015-0286-9es_ES
dc.relation.projectIDGobierno de España. AGL2011- 28904es_ES
dc.relation.projectIDGobierno de España. AGL2014-54089-Res_ES
dc.relation.projectIDGobierno de España. BES-2012-058642es_ES
dc.relation.projectIDGobierno de España. FPDI-2013-15619es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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