Mostrar el registro sencillo del ítem
Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis
dc.contributor.author | Ruiz, Pilar | |
dc.contributor.author | Causse, Manuel | |
dc.contributor.author | Vaquero Abellán, Manuel | |
dc.contributor.author | Casal Román, Manuel | |
dc.contributor.author | Gutiérrez-Aroca, Juan-Bautista | |
dc.date.accessioned | 2018-12-14T11:19:41Z | |
dc.date.available | 2018-12-14T11:19:41Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | http://hdl.handle.net/10396/17585 | |
dc.description.abstract | A new automated real-time PCR assay for the detection of rifampicin (RIF) and isoniazid (INH) resistance in Mycobacterium tuberculosis (MTB) was evaluated. A total of 163 clinical samples (128 pulmonary and 35 extra-pulmonary) were processed using four PCR assay kits: Abbott RealTime MTB RIF/INH, Genotype MTBDRplus, Xpert/MTB RIF, and Anyplex MTB/MDR. The results of phenotypic drug-susceptibility testing using BACTECMGIT 960 were used as reference. The sensitivity and specificity of the new Abbott RealTime MTB RIF/ INH assay in comparison with phenotypic testing was 96.3% (95%CI 87.32%–100%) for RIF and 100% (95%CI 99.3%–100%) for INH; the sensitivity was 78.8% (95%CI 66.8%–90.9%) and the specificity was 100% (95%CI 98.9%–100%). The Abbott RealTime MTB RIF/INH test could be a valid method for detecting the most common mutations in strains resistant to RIF and INH. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Dovepress | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Infection and Drug Resistance 10, 463–467 (2017) | es_ES |
dc.subject | Abbott RealTime MTB RIF/INH Resistance assay | es_ES |
dc.subject | M. tuberculosis | es_ES |
dc.subject | Mutations | es_ES |
dc.title | Evaluation of a new automated Abbott RealTime MTB RIF/INH assay for qualitative detection of rifampicin/isoniazid resistance in pulmonary and extra-pulmonary clinical samples of Mycobacterium tuberculosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.2147/IDR.S147272 | es_ES |
dc.relation.projectID | Gobierno de España. REIPI RD06/0008 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |