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dc.contributor.authorCuevas-Fernández, Beatriz
dc.contributor.authorFuentes Almagro, Carlos Alberto
dc.contributor.authorPeragón, Juan
dc.date.accessioned2019-03-08T11:06:57Z
dc.date.available2019-03-08T11:06:57Z
dc.date.issued2019
dc.identifier.urihttp://hdl.handle.net/10396/18070
dc.description.abstractLong-term starvation provokes a metabolic response in the brain to adapt to the lack of nutrient intake and to maintain the physiology of this organ. Here, we study the changes in the global proteomic profile of the rat brain after a seven-day period of food deprivation, to further our understanding of the biochemical and cellular mechanisms underlying the situations without food. We have used two-dimensional electrophoresis followed by mass spectrometry (2D-MS) in order to identify proteins differentially expressed during prolonged food deprivation. After the comparison of the protein profiles, 22 brain proteins were found with altered expression. Analysis by peptide mass fingerprinting and MS/MS (matrix-assisted laser desorption-ionization-time of flight mass spectrometer, MALDI-TOF/TOF) enabled the identification of 14 proteins differentially expressed that were divided into 3 categories: (1) energy catabolism and mitochondrial proteins; (2) chaperone proteins; and (3) cytoskeleton, exocytosis, and calcium. Changes in the expression of six proteins, identified by the 2D-MS proteomics procedure, were corroborated by a nanoliquid chromatography-mass spectrometry proteomics procedure (nLC-MS). Our results show that long-term starvation compromises essential functions of the brain related with energetic metabolism, synapsis, and the transmission of nervous impulse.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceNutrients 11(2), 219 (2019)es_ES
dc.subjectBraines_ES
dc.subjectProteomicses_ES
dc.subjectStarvationes_ES
dc.subject2-Des_ES
dc.subjectMitochondriaes_ES
dc.subjectCytoskeletones_ES
dc.titleProteomics Analysis Reveals the Implications of Cytoskeleton and Mitochondria in the Response of the Rat Brain to Starvationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/nu11020219es_ES
dc.relation.projectIDJunta de Andalucía. BIO-341es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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