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Activation of estrogen receptor-α induces gonadotroph progesterone receptor expression and action differently in young and middle-aged ovariectomized rats
dc.contributor.author | Gordón, Ana | |
dc.contributor.author | Aguilar, Rafaela | |
dc.contributor.author | Garrido, José | |
dc.contributor.author | Guil-Luna, Silvia | |
dc.contributor.author | Millán, Yolanda | |
dc.contributor.author | Martín de las Mulas González-Albo, Juana | |
dc.contributor.author | Sánchez Criado, J.E. | |
dc.date.accessioned | 2024-02-06T18:43:45Z | |
dc.date.available | 2024-02-06T18:43:45Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 02681161 | |
dc.identifier.uri | http://hdl.handle.net/10396/27188 | |
dc.description | Embargado hasta 01/01/2100 | |
dc.description.abstract | BACKGROUND: We attempted to define the effect of estrogen receptor (ER)α activation on gonadotroph progesterone receptor (PR) expression (mRNA and protein) and action (GnRH-stimulated and GnRH self-priming) in short- and long-term ovariectomized (OVX) rats.METHODSTwo weeks or 1 year after OVX, rats were injected over 3 days with 125 g/kg of estradiol benzoate (EB), 7.5 mg/kg of the selective ERα agonist propylpyrazole triol (PPT), or 15 mg/kg of the selective ER modulator tamoxifen (TX). Controls were given 0.2 ml oil. The last day of ER analog treatment, half of the rats in each group received 25 mg/kg of progesterone (P). The next day, anterior pituitaries were removed and analyzed for PR-AB mRNA and protein. Gonadotrophin secretion in incubated pituitaries was also measured. RESULTS(i):PR mRNA expression was higher in young than in middle-aged OVX rats although PR protein was absent in pituitaries from both groups of OVX rats; (ii) activation of ERα reduced gonadotroph hypertrophy and increased PR mRNA and protein expression (EB > PPT > TX) more efficiently in young than in middle-aged rats, (iii) ER agonists elicited GnRH-stimulated LH and FSH secretion in young but only FSH secretion in middle-aged OVX rats, (iv) evaluated by peak LH concentrations, GnRH self-priming was observed in both groups of OVX rats and (v) P down-regulated PR protein expression in young, and to a lesser extent, in middle-aged OVX rats, in close association with PR-dependent GnRH self-priming. CONCLUSIONS: Middle-aged OVX rats exhibited clear-cut LH, but not FSH, secretory defects in pituitary sensitivity to estrogen and P. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Oxford Academic | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Ana Gordon, Rafaela Aguilar, José C. Garrido-Gracia, Silvia Guil-Luna, Raquel Sánchez-Cespedes, Yolanda Millán, Juana Martín de las Mulas, José E. Sánchez-Criado, Activation of estrogen receptor-α induces gonadotroph progesterone receptor expression and action differently in young and middle-aged ovariectomized rats, Human Reproduction, Volume 24, Issue 10, October 2009, Pages 2618–2628, https://doi.org/10.1093/humrep/dep237 | es_ES |
dc.subject | Progesterone receptor expression | es_ES |
dc.subject | Gonadotrophin secretion | es_ES |
dc.subject | GnRH self-priming | es_ES |
dc.subject | Tamoxifen | es_ES |
dc.subject | Estrogen receptor-α agonists | es_ES |
dc.title | Activation of estrogen receptor-α induces gonadotroph progesterone receptor expression and action differently in young and middle-aged ovariectomized rats | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.1093/humrep/dep237 | es_ES |
dc.relation.projectID | Gobierno de España.MINECO BFU2008-01443 | es_ES |
dc.relation.projectID | Junta de Andalucía. CICE P07-CVI-2559 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.date.embargoEndDate | info:eu-repo/date/embargoEnd/2100-01-01 |