A single nucleotide polymorphism of the apolipoprotein A-V gene -1131T>C modulates postprandial lipoprotein metabolism

Abstract

The Apolipoprotein A–V (apoA–V) gene promoter polymorphism −1131T > C modulates triacylglycerol (TG) concentrations. We evaluate whether this polymorphism could be involved in the interindividual variability observed during postprandial lipemia.

Fifty-one healthy apo E3E3 male volunteers [12 with −1131CC/CT genotype, and 39 with −1131TT genotype] underwent a Vitamin A fat-load test consisting of 1 g of fat/kg body weight and 60,000 IU of Vitamin A. Blood samples were taken at time 0 and every hour until the 6th and every 2 h and 30 min until the 11th. Cholesterol (Chol) and TG were determined in plasma and Chol, TG, ApoB-100, ApoB-48, and retinyl palmitate (RP) were determined in lipoprotein fractions.

Data of postprandial lipemia revealed that subjects with the −1131CT/CC genotype had a higher postprandial response of total plasma TG (p = 0.043), large triacylglycerol-rich lipoproteins-TG (TRL-TG) (p = 0.002), large TRL-Chol (p = 0.004), small TRL-Chol (p = 0.004) and small TRL-RP (p = 0.001) than subjects with the −1131TT genotype.

The modifications observed in postprandial lipoprotein metabolism in subjects with the apoA–V −1131T > C polymorphism could be involved in the increased fasting plasma TG concentrations previously described in carriers of the C allele.