Spatial sap flow and xylem anatomical characteristics in olive trees under different irrigation regimes

Abstract

The compensation heat pulse method (CHP) is widely used to estimate sap flow and transpiration in conducting organs of woody plants. Previous studies have reported a natural azimuthal variability in sap flow, which could have practical implications in locating the CHP probes and integrating their output. Sap flow of several olive trees (Olea europaea L. cv. `Arbequina´) previously grown under different irrigation treatments were monitored by the CHP method, and their xylem anatomical characteristics were analyzed from wood samples taken at the same location in which the probes were installed. A significant azimuthal variability in the sap flow was found in a well irrigated olive tree monitored by eight CHP probes. The azimuthal variability was well related to crown architecture, but poorly to azimuthal differences in the xylem anatomical characteristics. Well-irrigated and deficit-irrigated olive trees showed similar xylem anatomical characteristics, but they differed in xylem growth, and in the ratio of nocturnal to diurnal sap flow (N/D index). The results of this work indicate transpiration could not be accurately estimated by the CHP method in olive trees if a small number of sensors are employed, and that the N/D index could be used as a sensitive water status indicator.