Preparation of porous methacrylate monoliths with oxidized single-walled carbon nanohorns for the extraction of nonsteroidal anti-inflammatory drugs from urine samples

Abstract

In this study we have developed a poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate-co-oxidized single walled carbon nanohorns) (poly(GMA-co-EDMA-co-o-SWNHs) spin columns via photo-polymerization for the extraction of nonsteroidal anti-inflammatory drugs (NSAIDs) in human urine samples. The microextraction procedure, including loading, washing and elution steps, was performed by centrifugation. Variables dealing with the covalent attachment of the hybrid monolith to polypropylene beds of the spin column device as well as variables related to the polymerization reaction were deeply studied. The resulting hybrid monolithic polymers were also characterized by scanning electron microscopy (SEM) and nitrogen intrusion porosimetry. A reversed-phase high-performance liquid chromatography (HPLC) with UV detection was used for quantitation of NSAIDs. The proposed methodology gave detection limits varied between 0.1 and 10 µg·L-1, and the precision (relative standard deviation) ranged from 3.5 and 11.8 %. Also, excellent relative recoveries values varied in the interval 81-106 % were found in the analysis of spiked urine samples.