El sistema mayor de histocompatibilidad en cabras (GLA). I. Prueba de microcitotoxicidad y obtención de sueros reactivos.

Abstract

Microcytotoxicity test to detect lymphocytes antigens in goats was described. According to our data, a two-step test, 1.077 Ficoll density and rabbit serum as the source of complement were the best procedures for detecting lymphocyte antigens in goats. Using this test and sera produced through skin grafting and immunizations with cells, we described two antisera (39 and 94) SC1 and SCz that detect two antigens or two groups of antigens of the histocompatibility complex in goats (GLA). These antisera, with title of 1/128 and 1/256 respectively, react with cells from differents animals presenting clear cut behaviours: SCl recognized 14% positives animals and SC 2,42%. The sera activity lasted for three months after the final inoculation.

Se ha realizado la puesta a punto de una prueba de microcitotoxicidad para detectar antígenos linfocitarios en cabras. Mediante esta dócima y utilizando sueros producidos por trasplantes de piel en cabras, se describen dos antisueros (SC1 y SC2 ), capaces de detectar distintos antígenos o grupos de antígenos pertenecientes al sistema de histocompatibilidad caprino (GLA).