First Report of Southern Blight of Pepper Caused by Sclerotium rolfsii in Southern Spain

Abstract

In May 2009, a stem rot of pepper (Capsicum annuum L.) occurred in a 20-ha field in Hacienda de Tarazona, Seville, in southern Spain. Affected plants appeared singly or were grouped in circular patches as much as 8 to 10 m in diameter. Early symptoms consisted of water-soaked lesions on crown and lower stem tissue in contact with the soil. Plant foliage became pale green and wilted, followed by a complete collapse of the plant. A dense white mycelial mat formed on the lower stem and crown with 1- to 2-mm-diameter, spherical, tan-to-dark brown sclerotia. Lower stem pieces of 12 plants with early disease symptoms were surface sterilized in 0.5% NaOCl, dried, transferred to acidified potato dextrose agar, and incubated at 25 ± 1°C in the dark. Fast-growing fungal colonies with white mycelium and abundant sclerotia developed after 6 to 10 days of incubation. On the basis of morphological characters, the fungus was identified as Sclerotium rolfsii Sacc. (2). To confirm the identity of the pathogen, the ribosomal DNA internal transcribed spacer was amplified and sequenced for two isolates (one of the two exact sequences was deposited as GenBank Accession No. GU080230). The sequence was 99% similar to sequences of Athelia rolfsii (S. rolfsii) in GenBank. Pathogenicity of two isolates was determined by placing two oat seeds colonized by each isolate 0.5 to 1 cm from the stem of 2-week-old pepper plants cv. Cristal (one plant per pot, eight replicates). Plants were incubated in a growth chamber maintained at 28 ± 1°C with a 14-h photoperiod of 360 μE·m–2·s–1 and 60 to 90% relative humidity for 10 days. By the sixth day, discoloration and blight of the foliage and stem was observed. Sclerotia formed around the crown and 88% of the plants died 7 days after inoculation. S. rolfsii was recovered from all affected pepper plants. Noninoculated control plants did not develop symptoms. In southern Spain, S. rolfsii is widely distributed in areas of sugar beet production (1). Because of the wide host range of the pathogen, southern blight could become an important disease of vegetable production in southern Spain.