Kahm, Matthias
Navarrete, Clara
Llopis-Torregrosa, Vicent
Herrera, Rito
Barreto, Lina
Yenush, Lynne
Ariño, Joaquín
Ramos, José
Kschischo, Maik
2013-12-23T09:35:32Z
2013-12-23T09:35:32Z
2012
http://hdl.handle.net/10396/11517
The intrinsic ability of cells to adapt to a wide range of environmental conditions is a fundamental process required for
survival. Potassium is the most abundant cation in living cells and is required for essential cellular processes, including the
regulation of cell volume, pH and protein synthesis. Yeast cells can grow from low micromolar to molar potassium
concentrations and utilize sophisticated control mechanisms to keep the internal potassium concentration in a viable range.
We developed a mathematical model for Saccharomyces cerevisiae to explore the complex interplay between biophysical
forces and molecular regulation facilitating potassium homeostasis. By using a novel inference method (‘‘the reverse
tracking algorithm’’) we predicted and then verified experimentally that the main regulators under conditions of potassium
starvation are proton fluxes responding to changes of potassium concentrations. In contrast to the prevailing view, we show
that regulation of the main potassium transport systems (Trk1,2 and Nha1) in the plasma membrane is not sufficient to
achieve homeostasis
application/pdf
eng
Public Libray of Science (PLOS)
https://creativecommons.org/licenses/by-nc-nd/4.0/
PLoS Computational Biology 8 (6) (2012)
Potassium
Mathematical model
Saccharomyces cerevisiae
Potassium Starvation in Yeast: Mechanisms of Homeostasis Revealed by Mathematical Modeling
info:eu-repo/semantics/article
info:eu-repo/semantics/openAccess