Valhondo, Isabel
Hassouneh, Fakhri
López-Sejas, Nelson
Pera Rojas, Alejandra
Sánchez-Correa, Beatriz
Guerrero, Beatriz
Bergua, Juan M.
Arcos, María José
Bañas, Helena
Casas-Avilés, Ignacio
Sánchez García, Joaquín
Serrano, Josefina
Martín, Carmen
Durán, Esther
Alonso, Corona
Solana Lara, Rafael
Tarazona, Raquel
2020-08-05T09:51:07Z
2020-08-05T09:51:07Z
2020
http://hdl.handle.net/10396/20400
Background: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56− T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56− T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1−TIGIT+TACTILE+ NK cells and DNAM-1− TIGIT+TACTILE+ CD56− T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade.
application/pdf
eng
MDPI
https://creativecommons.org/licenses/by/4.0/
Cancers 12(8), 2171 (2020)
Acute myeloid leukemia
DNAM-1
NK cells
NKT-like cells
TACTILE
T cells
TIGIT
Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia
info:eu-repo/semantics/article
http://dx.doi.org/10.3390/cancers12082171
Gobierno de España. SAF2017-87538-R
Instituto de Salud Carlos III. PI16/01615
Instituto de Salud Carlos III. CP19/00008
info:eu-repo/semantics/openAccess