Medrano-Padial, Concepción Puerto, María Prieto, Ana Isabel Ayala-Soldado, Nahúm Beaumont, Pauline Rouger, Caroline Krisa, Stéphanie Pichardo, Silvia 2024-01-17T13:21:05Z 2024-01-17T13:21:05Z 2021 http://hdl.handle.net/10396/26594 Genotoxic data of substances that could be used as food additives are required by the European Food Safety Authority. In this sense, the use of an extract from grapevine shoots containing a stilbene richness of 99% (ST-99), due to its antioxidant and antibacterial activities, has been proposed as an alternative to sulfur dioxide in wine. The aim of this work was to study, for the first time, the in vivo genotoxic effects produced in rats orally exposed to 90, 180, or 360 mg ST-99/kg body weight at 0, 24, and 45 h. The combination of micronucleus assay in bone marrow (OECD 474) and standard (OECD 489) and enzyme-modified comet assay was used to determine the genotoxicity on cells isolated from stomach, liver, and blood of exposed animals. The ST-99 revealed no in vivo genotoxicity. These results were corroborated by analytical studies that confirm the presence of stilbenes and their metabolites in plasma and tissues. Moreover, to complete these findings, a histopathological study was performed under light microscopy in liver and stomach showing only slight modifications in both organs at the highest concentration used. The present work confirms that this extract is not genotoxic presenting a good profile for its potential application as a preservative in the wine industry. application/pdf eng MDPI https://creativecommons.org/licenses/by/4.0/ Medrano-Padial, C., Puerto, M., Prieto, A. I., Ayala, N., Beaumont, P., Rouger, C., Krisa, S., & Pichardo, S. (2021). In vivo genotoxicity evaluation of a Stilbene extract prior to its use as a natural additive: a combination of the micronucleus test and the comet assay. Foods, 10(2), 439. https://doi.org/10.3390/foods10020439 Stilbenes Genotoxicity Comet assay Micronucleus In vivo Rat In Vivo Genotoxicity Evaluation of a Stilbene Extract Prior to Its Use as a Natural Additive: A Combination of the Micronucleus Test and the Comet Assay info:eu-repo/semantics/article https://doi.org/10.3390/foods10020439 Gobierno de España. RTA2015-00005-C02-02 info:eu-repo/semantics/openAccess