Barilli, Eleonora Rubiales, Diego Castillejo Sanchez, Maria Angeles 2025-01-13T15:49:07Z 2025-01-13T15:49:07Z 2012 http://hdl.handle.net/10396/30885 Systemic acquired resistance (SAR) to Uromyces pisi in pea was studied by using a proteomic approach. Two-dimensional electrophoresis (2-DE) was used in order to compare the leaf proteome of two pea genotypes displaying different phenotypes (susceptible and partial resistance to the fungus), and in response to parasite infection under the effect of two inducers of SAR, BTH and BABA. Multivariate statistical analysis identified 126 differential protein spots under the experimental conditions (genotypes/treatments). All of these 126 protein spots were subjected to MALDI-TOF/TOF mass spectrometry to deduce their possible functions. A total of 50 proteins were identified using a combination of peptide mass fingerprinting (PMF) and MSMS fragmentation. Most of the identified proteins corresponded to enzymes belonging to photosynthesis, metabolism, biosynthesis, binding and defense response, whose behavior pattern was different in relation to susceptibility/ resistance of the genotypes studied and to the BTH/BABA induction to pathogen response. Results obtained in this work suggested that plants could reduce their photosynthesis and other energy metabolism and enhance the production of defense-related proteins to cope the stress. On the other side, we postulated that resistance induced by the chemicals operates via different mechanisms: BABA inducer could act via phenolic biosynthesis pathway, whereas resistance provided by BTH inducer seems to be mediated by defense and stress-related proteins. The results are discussed in terms of response to rust under the effect of inducers. application/pdf eng Elsevier https://creativecommons.org/licenses/by-nc-nd/4.0/ Eleonora Barilli, Diego Rubiales, Mª. Ángeles Castillejo, Comparative proteomic analysis of BTH and BABA-induced resistance in pea (Pisum sativum) toward infection with pea rust (Uromyces pisi), Journal of Proteomics, Volume 75, Issue 17, 2012. Proteomics Pisum sativum Uromyces pisi Systemic acquired resistance Benzothiadiazole 3-Aminobutyric acid Comparative proteomic analysis of BTH and BABA-induced resistance in pea (Pisum sativum) toward infection with pea rust (Uromyces pisi) info:eu-repo/semantics/article https://doi.org/10.1016/j.jprot.2012.06.033 info:eu-repo/semantics/openAccess