Adaptation to potassium starvation of wild-type and K+-transport mutant (trk1,2) of Saccharomyces cerevisiae: 2-dimensional gel electrophoresis-based proteomic approach
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Author
Gelis, Samuel
Curto Rubio, Miguel
Valledor, Luis
González, Asier
Ariño, Joaquín
Jorrín-Novo, Jesús V.
Ramos, José
Publisher
Blackwell Publishing LtdDate
2012Subject
2D-gelsPotassium homeostasis
Sacharomyces cerevisae
TRK1
TRK2
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Show full item recordAbstract
Saccharomyces cerevisiaewild-type (BY4741) and the correspondingmutant lacking
the plasma membrane main potassium uptake systems (trk1,trk2) were used to
analyze the consequences of K+ starvation following a proteomic approach. In
order to trigger high-affinity mode of potassium transport, cells were transferred to
potassium-freemedium.Protein profilewas followed by two-dimensional (2-D) gels
in samples taken at 0, 30, 60, 120, 180, and 300 min during starvation.We observed
a general decrease of protein content during starvation that was especially drastic
in the mutant strain as it was the case of an important number of proteins involved
in glycolysis. On the contrary, we identified proteins related to stress response and
alternative energetic metabolism that remained clearly present. Neural networkbased
analysis indicated that wild type was able to adapt much faster than the
mutant to the stress process. We conclude that complete potassium starvation is a
stressful process for yeast cells, especially for potassium transport mutants, and we
propose that less stressing conditions should be used in order to study potassium
homeostasis in yeast.