Short communication. In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed
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Author
Demyda-Peyrás, S.
Hidalgo, Manuel
Dorado, Jesús
Anter, J.
Género, Enrique Rubén
Moreno-Millán, M.
Publisher
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Date
2013Subject
Breed effectBullfight
in vitro fertilization
Lidia cattle breed
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Show full item recordAbstract
The Lidia bovine breed is the most successful cattle breed on the Iberian Peninsula, also considered a hallmark of
Spanish tradition and image around the world. The aims of the study were to characterize the oocyte recovery rates
and to evaluate the effect of two standard in vitro maturation protocols on oocyte maturation (cumulus expansion and
nuclear maturation) and fertilization rates after in vitro fertilization in this breed. For this purpose, 261 ovaries from
Lidia cows were processed obtaining 1,125 viable cumulus oocyte complexes (COCs). The oocyte recovery rate obtained
(4.31 viable COCs per ovary) was lower than those described previously in other studied breeds. Maturation rates were
evaluated in two different oocyte maturation media with (M1) and without (M2) hormonal supplementation. The
percentage of COCs with expanded cumulus cells was significantly lower in M1 (74.35%) compared with M2 (82.25%).
Metaphase II (MII) rates (67.75% in M1 and 73.18% in M2) were similar to previous studies in different cattle
populations. M2 significantly improved the percentage of COCs with their cumulus cells expanded (p < 0.01) and
nuclear maturation rates (p < 0.05), but it did not affect the fertilization percentages obtained in this experiment. In
conclusion, our study suggests that oocytes of the Lidia cattle breed can be obtained, matured and fertilized following
standard protocols previously used in other cattle breeds.