Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization
Autor
Luna, Diego
Bautista, Felipa M.
Luna, Carlos
Calero, Juan
Posadillo, Alejandro
Romero, Antonio A.
Sancho, Enrique D.
Estévez, R.
Editor
MDPIFecha
2017Materia
BiodieselEcodiesel
Lipases
CALB
Enzymatic extracts
Transesterification
Glycerol
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The application of biocatalysts in the transesterification process of triglycerides (TG) allows
integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the
environmental sustainability of the conventional biodiesel production process. This is known as
Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified
commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the
same species of commercial lipases currently applied in this process is being investigated. Thus,
Candida antarctica type B (CALB) was chosen to determine the optimal conditions of culture of
this yeast. The standard strain was obtained from the Spanish Type Microbial Cultures Collection
(CECT) and has been used to carry out several studies to elucidate its optimum growth conditions.
Through a process of lyophilization with prior dialysis of the liquid cultures, the enzymatic extracts
were obtained. The different obtained cultures have been applied as biocatalysts in the 1,3-selective
transesterification reaction of sunflower oil with ethanol to obtain Ecodiesel (FAEE + MG). Selectivity
and reaction yields were obtained by gas chromatography. Acceptable yields are obtained during the
reaction time as well as in successive reactions, demonstrating the feasibility of using these CALB
enzymatic extracts as biocatalysts