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Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia
dc.contributor.author | Valhondo, Isabel | |
dc.contributor.author | Hassouneh, Fakhri | |
dc.contributor.author | López-Sejas, Nelson | |
dc.contributor.author | Pera Rojas, Alejandra | |
dc.contributor.author | Sánchez-Correa, Beatriz | |
dc.contributor.author | Guerrero, Beatriz | |
dc.contributor.author | Bergua, Juan M. | |
dc.contributor.author | Arcos, María José | |
dc.contributor.author | Bañas, Helena | |
dc.contributor.author | Casas-Avilés, Ignacio | |
dc.contributor.author | Sánchez García, Joaquín | |
dc.contributor.author | Serrano, Josefina | |
dc.contributor.author | Martín, Carmen | |
dc.contributor.author | Durán, Esther | |
dc.contributor.author | Alonso, Corona | |
dc.contributor.author | Solana Lara, Rafael | |
dc.contributor.author | Tarazona, Raquel | |
dc.date.accessioned | 2020-08-05T09:51:07Z | |
dc.date.available | 2020-08-05T09:51:07Z | |
dc.date.issued | 2020 | |
dc.identifier.uri | http://hdl.handle.net/10396/20400 | |
dc.description.abstract | Background: Acute myeloid leukemia (AML) remains a major clinical challenge due to poor overall survival, which is even more dramatic in elderly patients. TIGIT, an inhibitory receptor that interacts with CD155 and CD112 molecules, is considered as a checkpoint in T and NK cell activation. This receptor shares ligands with the co-stimulatory receptor DNAM-1 and with TACTILE. The aim of this work was to analyze the expression of DNAM-1, TIGIT and TACTILE in NK cells and T cell subsets in AML patients. Methods: We have studied 36 patients at the time of diagnosis of AML and 20 healthy volunteers. The expression of DNAM-1, TIGIT and TACTILE in NK cells and T cells, according to the expression of CD3 and CD56, was performed by flow cytometry. Results: NK cells, CD56− T cells and CD56+ T (NKT-like) cells from AML patients presented a reduced expression of DNAM-1 compared with healthy volunteers. An increased expression of TIGIT was observed in mainstream CD56− T cells. No differences were observed in the expression of TACTILE. Simplified presentation of incredibly complex evaluations (SPICE) analysis of the co-expression of DNAM-1, TIGIT and TACTILE showed an increase in NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE. Low percentages of DNAM-1−TIGIT+TACTILE+ NK cells and DNAM-1− TIGIT+TACTILE+ CD56− T cells were associated with a better survival of AML patients. Conclusions: The expression of DNAM-1 is reduced in NK cells and in CD4+ and CD8+ T cells from AML patients compared with those from healthy volunteers. An increased percentage of NK and T cells lacking DNAM-1 and co-expressing TIGIT and TACTILE is associated with patient survival, supporting the role of TIGIT as a novel candidate for checkpoint blockade. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.source | Cancers 12(8), 2171 (2020) | es_ES |
dc.subject | Acute myeloid leukemia | es_ES |
dc.subject | DNAM-1 | es_ES |
dc.subject | NK cells | es_ES |
dc.subject | NKT-like cells | es_ES |
dc.subject | TACTILE | es_ES |
dc.subject | T cells | es_ES |
dc.subject | TIGIT | es_ES |
dc.title | Characterization of the DNAM-1, TIGIT and TACTILE Axis on Circulating NK, NKT-Like and T Cell Subsets in Patients with Acute Myeloid Leukemia | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.3390/cancers12082171 | es_ES |
dc.relation.projectID | Gobierno de España. SAF2017-87538-R | es_ES |
dc.relation.projectID | Instituto de Salud Carlos III. PI16/01615 | es_ES |
dc.relation.projectID | Instituto de Salud Carlos III. CP19/00008 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |