Plant Defensins NaD1 and NaD2 Induce Different Stress Response Pathways in Fungi
Autor
Dracatos, Peter M.
Payne, Jennifer
Anderson, Marilyn A.
Plummer, Kim M.
Di Pietro, Antonio
Editor
MDPIFecha
2016Materia
Antifungal defensinsNaD1 and NaD2
CWI signalling pathway
HOG signalling pathway
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Nicotiana alata defensins 1 and 2 (NaD1 and NaD2) are plant defensins from the ornamental
tobacco that have antifungal activity against a variety of fungal pathogens. Some plant defensins
interact with fungal cell wall O-glycosylated proteins. Therefore, we investigated if this was
the case for NaD1 and NaD2, by assessing the sensitivity of the three Aspergillus nidulans (An)
O-mannosyltransferase (pmt) knockout (KO) mutants (AnDpmtA, AnDpmtB, and AnDpmtC). AnDpmtA
was resistant to both defensins, while AnDpmtC was resistant to NaD2 only, suggesting NaD1 and
NaD2 are unlikely to have a general interaction with O-linked side chains. Further evidence of this
difference in the antifungal mechanism was provided by the dissimilarity of the NaD1 and NaD2
sensitivities of the Fusarium oxysporum f. sp. lycopersici (Fol) signalling knockout mutants from the cell
wall integrity (CWI) and high osmolarity glycerol (HOG) mitogen-activated protein kinase (MAPK)
pathways. HOG pathway mutants were sensitive to both NaD1 and NaD2, while CWI pathway
mutants only displayed sensitivity to NaD2