Mostrar el registro sencillo del ítem
An AP Endonuclease Functions in Active DNA Demethylation and Gene Imprinting in Arabidopsis
dc.contributor.author | Li, Yan | |
dc.contributor.author | Córdoba-Cañero, Dolores | |
dc.contributor.author | Qian, Weiqiang | |
dc.contributor.author | Zhu, Xiaohong | |
dc.contributor.author | Tang, Kai | |
dc.contributor.author | Zhang, Huiming | |
dc.contributor.author | Rodríguez Ariza, Rafael | |
dc.contributor.author | Roldán-Arjona, Teresa | |
dc.contributor.author | Zhu, Jian-Kang | |
dc.date.accessioned | 2017-12-15T08:08:09Z | |
dc.date.available | 2017-12-15T08:08:09Z | |
dc.date.issued | 2015 | |
dc.identifier.uri | http://hdl.handle.net/10396/15734 | |
dc.description.abstract | Active DNA demethylation in plants occurs through base excision repair, beginning with removal of methylated cytosine by the ROS1/DME subfamily of 5-methylcytosine DNA glycosylases. Active DNA demethylation in animals requires the DNA glycosylase TDG or MBD4, which functions after oxidation or deamination of 5-methylcytosine, respectively. However, little is known about the steps following DNA glycosylase action in the active DNA demethylation pathways in plants and animals. We show here that the Arabidopsis APE1L protein has apurinic/apyrimidinic endonuclease activities and functions downstream of ROS1 and DME. APE1L and ROS1 interact in vitro and co-localize in vivo. Whole genome bisulfite sequencing of ape1l mutant plants revealed widespread alterations in DNA methylation. We show that the ape1l/zdp double mutant displays embryonic lethality. Notably, the ape1l+/2zdp2/2 mutant shows a maternal-effect lethality phenotype. APE1L and the DNA phosphatase ZDP are required for FWA and MEA gene imprinting in the endosperm and are important for seed development. Thus, APE1L is a new component of the active DNA demethylation pathway and, together with ZDP, regulates gene imprinting in Arabidopsis. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | PLoS Genetics 11(1): e1004905 (2015) | es_ES |
dc.subject | DNA methylation | es_ES |
dc.subject | Seeds | es_ES |
dc.subject | Endosperm | es_ES |
dc.subject | DNA | es_ES |
dc.subject | Arabidopsis thaliana | es_ES |
dc.subject | Mutation | es_ES |
dc.subject | Plant genomics | es_ES |
dc.subject | Genomic imprinting | es_ES |
dc.title | An AP Endonuclease Functions in Active DNA Demethylation and Gene Imprinting in Arabidopsis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pgen.1004905 | es_ES |
dc.relation.projectID | Instituto de Salud Carlos III. R01GM070795 | es_ES |
dc.relation.projectID | Instituto de Salud Carlos III. R01GM059138 | es_ES |
dc.relation.projectID | Gobierno de España. BFU2013-43269-P | es_ES |
dc.relation.projectID | Junta de Andalucía. P11-CVI-7576 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |