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Direct Determination of Glutathione S-transferase and Glucose-6-phosphate Dehydrogenase Activities in Cells Cultured in Microtitre Plates as Biomarkers for Oxidative Stress
(Fund for the Replacement of Animals in Medical Experiments, 1998)
The enzymes glutathione S,-transferase (GST) and glucose-6-phosphate dehydrogenase
(G-6PDH) are implicated in the defence against oxidative stress. GST is mainly involved
in the conjugation of electrophilic compounds ...
Molecular basis for the distinct divalent cation requirement in the uridylylation of the signal transduction proteins GlnJ and GlnB from Rhodospirillum rubrum
(BioMed Central, 2012)
Background: PII proteins have a fundamental role in the control of nitrogen metabolism in bacteria, through
interactions with different PII targets, controlled by metabolite binding and post-translational modification,
...
Expresión del gen chit42 de Trichoderma harzianum en plantas de fresa: obtención de plantas de fresa con mayor resistencia a la infección por Colletrotrichum acutatum
(Universidad de Córdoba, 2009)
La antracnosis, causada por diferentes especies del hongo Colletotrichum, origina importantes pérdidas de
producción en el cultivo de la fresa, un cultivo de gran interés agrícola en Andalucía, que aporta el 92.01% de
la ...
Biología molecular de la asimilación de nitrato en algas
(Universidad de Córdoba, 2009)
El alga verde eucariótica Chlamydomonas reinhardtii es un organismo modelo para el estudio de la biología
molecular de la asimilación de nitrato. Este sistema biológico presenta excelentes cualidades para estudios
bioquímicos, ...
Varietal Tracing of Virgin Olive Oils Based on Plastid DNA Variation Profiling
(Public Library of Science, 2013)
Olive oil traceability remains a challenge nowadays. DNA analysis is the preferred approach to an effective varietal
identification, without any environmental influence. Specifically, olive organelle genomics is the most ...
Low oxygen levels contribute to improve photohydrogen production in mixotrophic non‑stressed Chlamydomonas cultures
(BioMed Central, 2015)
Background: Currently, hydrogen fuel is derived mainly from fossil fuels, but there is an increasing interest in clean
and sustainable technologies for hydrogen production. In this context, the ability of some photosynthetic ...
Arginine is a component of the ammonium- CYG56 signalling cascade that represses genes of the nitrogen assimilation pathway in Chlamydomonas reinhardtii
(Public Library of Science, 2018)
Nitrogen assimilation and metabolism are essential processes for all living organisms, yet
there is still much to be learnt on how they are regulated. The use of Chlamydomonas reinhardtii
as a model system has been ...
Characterization of a Mutant Deficient for Ammonium and Nitric Oxide Signalling in the Model System Chlamydomonas reinhardtii
(Public Library of Science, 2016)
The ubiquitous signalling molecule Nitric Oxide (NO) is characterized not only by the variety
of organisms in which it has been described, but also by the wealth of biological processes
that it regulates. In contrast to ...
Glucose Uptake and Its Effect on Gene Expression in Prochlorococcus
(Public Library Of Science (PLOS), 2008)
The marine cyanobacteria Prochlorococcus have been considered photoautotrophic microorganisms, although the
utilization of exogenous sugars has never been specifically addressed in them. We studied glucose uptake in ...
Direct Determination of Glutathione Reductase in Cells Cultured in Microtitre Plates as a Biomarker for Oxidative Stress
(Fund for the Replacement of Animals in Medical Experiments, 1995)
A new method was developed for the direct determination of glutathione reductase
(GOR) activity in Vero cells cultured in microtitre plates, avoiding cell-free extract preparation.
The cells in each well were washed twice ...