DNA fragmentation of equine cumulus cells from Cumulus-Oocyte complexes submitted to vitrification and its relationship to the developmental competence of the oocyte
Autor
Ortiz, I.
Dorado, Jesús
Pereira, Blasa
Díaz-Jiménez, María
Consuegra, César
Gosalvez, Jaime
Hidalgo, Manuel
Editor
WileyFecha
2022Materia
Cumulus cellsDNA fragmentation
Equines
Oocyte vitrification
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The objectives of this study were to evaluate the effect of vitrification on the DNA fragmentation rate of equine cumulus cells and to assess its relationship to oocyte in vitro maturation (IVM) after vitrification. Cumulus cells (CC) from 14 mares were recovered from COCs, previously submitted to vitrification (VIT) and IVM. The DNA fragmentation rate of the cumulus cells (CC-DF) was assessed using a chromatin dis-persion test. CC-DF rates between vitrified and control COCs were statistically com-pared by Student’s t- test. The rates of CC-DF from control COCs were lower than in vitrified COCs. The percentage of CC-DF was not significantly different (p> .05) be-tween groups of COCs able to reach metaphase II (MII > 0) and those in which oocyte maturation was not achieved (MII = 0). In conclusion, vitrification has a deleterious effect on the DNA fragmentation of equine cumulus cells; however, this parameter cannot be used as a predictor for IVM success after COCs vitrification.