Listar por autor "1b3cfb5d-6e72-4f72-a8d7-1bc99c2306ba"
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Alternative pathway for 3-cyanoalanine assimilation in pseudomonas pseudoalcaligenes CECT5344 under Noncyanotrophic conditions
Pérez, María Dolores; Olaya-Abril, Alfonso; Cabello, Purificación; Sáez, Lara P.; Roldán, María Dolores; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel (American Society for Microbiology, 2021)3-Cyanoalanine and cyanohydrins are intermediate nitriles produced in cyanide degradation pathways in plants and bacteria. 3-Cyanoalanine is generated from cyanide by the 3-cyanoalanine synthase, an enzyme mainly characterized ... -
Assimilation of cyanide and cyano-derivatives by Pseudomonas pseudoalcaligenes CECT5344: from omic approaches to biotechnological applications
Cabello, Purificación; Luque-Almagro, Víctor Manuel; Olaya-Abril, Alfonso; Sáez, Lara P.; Moreno-Vivián, Conrado; Roldán, María Dolores (Oxford Academic, 2018)Mining, jewellery and metal-processing industries use cyanide for extracting gold and other valuable metals, generating large amounts of highly toxic wastewater. Biological treatments may be a clean alternative under the ... -
Bioremediation of cyanide-containing wastes: The potential of systems and synthetic biology for cleaning up the toxic leftovers from mining
Roldán Alfonso, María Dolores; Olaya-Abril, Alfonso; Sáez, Lara P.; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Moreno-Vivián, Conrado (Wiley, 2021)Cyanide is a highly toxic chemical produced in large amounts by the mining and jewellery industries, steel manufacturing, coal coking, food processing and chemical synthesis (Luque-Almagro et al, 2011). The mining industry ... -
Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster
Sáez, Lara P.; Cabello, Purificación; Ibáñez, María Isabel; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Moreno-Vivián, Conrado (MDPI, 2019)The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 can grow with cyanate, cyanide, or cyanide-containing industrial residues as the sole nitrogen source, but the assimilation of cyanide and cyanate takes ... -
DNA microarray analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to nitrogen starvation, cyanide and a jewelry wastewater
Luque-Almagro, Víctor Manuel; Escribano Fernández, María de la Paz; Manso Cobos, Isabel; Sáez, Lara P.; Cabello, Purificación; Roldán, María Dolores; Moreno-Vivián, Conrado (Elsevier, 2015)tPseudomonas pseudoalcaligenes CECT5344 is an alkaliphilic bacterium that can use cyanide as nitrogensource for growth, becoming a suitable candidate to be applied in biological treatment of cyanide-containing wastewaters. ... -
Exploring anaerobic environments for cyanide and cyano-derivatives microbial degradation
Luque-Almagro, Víctor Manuel; Cabello, Purificación; Sáez, Lara P.; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado; Roldán, María Dolores (SpringerLink, 2018)Cyanide is one of the most toxic chemicals for living organisms described so far. Its toxicity is mainly based on the high affinity that cyanide presents toward metals, provoking inhibition of essential metalloenzymes. ... -
Genomic Insights into Cyanide Biodegradation in the Pseudomonas Genus
Sáez, Lara P.; Rodríguez Caballero, Gema; Olaya-Abril, Alfonso; Cabello, Purificación; Moreno-Vivián, Conrado; Roldán, María Dolores; Luque-Almagro, Víctor Manuel (MDPI, 2024)Molecular studies about cyanide biodegradation have been mainly focused on the hydrolytic pathways catalyzed by the cyanide dihydratase CynD or the nitrilase NitC. In some Pseudomonas strains, the assimilation of cyanide ... -
Holistic view of biological nitrogen fixation and phosphorus mobilization in Azotobacter chroococcum NCIMB 8003
Biełło, Karolina A.; Lucena, Carlos; López Tenllado, Francisco Javier; Hidalgo-Carrillo, J.; Rodríguez Caballero, Gema; Cabello, Purificación; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Moreno-Vivián, Conrado; Olaya-Abril, Alfonso (Frontiers, 2023)Nitrogen (N) and phosphorus (P) deficiencies are two of the most agronomic problems that cause significant decrease in crop yield and quality. N and P chemical fertilizers are widely used in current agriculture, causing ... -
Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Roldán, María Dolores; Olaya-Abril, Alfonso; Moreno-Vivián, Conrado (MDPI, 2023)Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the ... -
Putative small RNAs controlling detoxification of industrial cyanide-containing wastewaters by Pseudomonas pseudoalcaligenes CECT5344
Olaya-Abril, Alfonso; Luque-Almagro, Víctor Manuel; Pérez, María Dolores; López, Cristina María; Amil-Ruiz, Francisco; Cabello, Purificación; Sáez, Lara P.; Moreno-Vivián, Conrado; Roldán, María Dolores (PLOS, 2019)The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 uses free cyanide and several metal−cyanide complexes as the sole nitrogen source and tolerates high concentrations of metals like copper, zinc and iron, ... -
Quantitative proteomic analysis of cyanide and mercury detoxification by pseudomonas pseudoalcaligenes CECT 5344
Biełło, Karolina A.; Olaya-Abril, Alfonso; Cabello, Purificación; Rodríguez Caballero, Gema; Sáez, Lara P.; Moreno-Vivián, Conrado; Luque-Almagro, Víctor Manuel; Roldán, María Dolores (American Society for Microbiology, 2023)The cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT 5344 uses cyanide and different metal-cyanide complexes as the sole nitrogen source. Under cyanotrophic conditions, this strain was able to grow with up ... -
Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-contain ing wastewaters using Liquid Chromatography- Mass Spectrometry/Mass Spectrometry (LC- MS/MS)
Ibáñez, María Isabel; Cabello, Purificación; Luque-Almagro, Víctor Manuel; Sáez, Lara P.; Olaya-Abril, Alfonso; Sánchez de Medina, Verónica; Luque de Castro, M.D.; Moreno-Vivián, Conrado; Roldán, María Dolores (PLOS, 2017)Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas ps ... -
Role of the dihydrodipicolinate synthase DapA1 on iron homeostasis during cyanide assimilation by the alkaliphilic bacterium pseudomonas pseudoalcaligenes CECT5344
Olaya-Abril, Alfonso; Pérez, María Dolores; Cabello, Purificación; Martignetti, Diego; Sáez, Lara P.; Luque-Almagro, Víctor Manuel; Moreno-Vivián, Conrado; Roldán, María Dolores (Frontiers, 2020)Cyanide is a toxic compound widely used in mining and jewelry industries, as well as in the synthesis of many different chemicals. Cyanide toxicity derives from its high affinity for metals, which causes inhibition of ...