Determinaçao simultânea de resíduos de cloralfenicol, trianfenicol e florfenicol em peite bovino por cromatografia electrocinética micelar
Simultaneous determination of chloroamphenicl, thianphenicl and florfenicl residues in bovine milk by micellar electrokinetic chromatography
Autor
Pezza, Leonardo
Ríos, Ángel
Nozal, Leonor
Arce Jiménez, Lourdes
Valcárcel, M.
Editor
Sociedade Brasileira de QuimicaFecha
2006Materia
Micelar electrokinetic chromatographyAmphenicols
Milk
METS:
Mostrar el registro METSPREMIS:
Mostrar el registro PREMISMetadatos
Mostrar el registro completo del ítemResumen
A micellar electrokinetic chromatographic
method (MEKC) is described for determining residues of amphenicols(chloramphenicol,thiamphenicol and florfenicol) in bovine
milk. MEKC is conducted by using a separation buffer consisting of 20 mM Na2HPO4, 10 mM Na2B4O7, 50 mM SDS at pH 8.0;
UV detection at 210 nm and 10 kV of voltage. The limit of detection ranged from 4.3-5.3 μg L-1. The MEKC method was
applied for the simultaneous determination of amphenicols in milk samples spiked with amphenicols at three concentration
levels: 10, 30 and 50 μg L-1. Recoveries ranging from 91-105% were obtained by following a simple extraction/preconcentration
procedure.