Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase
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Autor
Luna, Carlos
Verdugo-Escamilla, Cristóbal
Sancho, Enrique D.
Luna, Diego
Calero, Juan
Posadillo, Alejandro
Bautista, Felipa M.
Romero, Antonio A.
Editor
SpringerOpenFecha
2014Materia
BiodieselEnzyme biocatalysis
Candida antarctica lipase B (CALB)
N435
Response surface methodology
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Background: Novozym 435, a commercial lipase from Candida antarctica, recombinant, expressed in Aspergillus niger,
immobilized on macroporous acrylic resin, has been already described in the obtention of biodiesel. It is here evaluated
in the production of a new biofuel that integrates the glycerol as monoglyceride (MG) together with two fatty acid
ethyl esters (FAEE) molecules by the application of 1,3-selective lipases in the ethanolysis reaction of sunflower oil.
Results: Response surface methodology (RSM) is employed to estimate the effects of main reaction. Optimum
conditions for the viscosity, selectivity, and conversion were determined using a multifactorial design of
experiments with three factors run by the software Stat Graphics version XV.I. The selected experimental
parameters were reaction temperature, oil/ethanol ratio and alkaline environment. On the basis of RSM analysis,
the optimum conditions for synthesis were 1/6 oil/EtOH molar ratio, 30°C, and 12.5 μl of NaOH 10 N aqueous
solutions, higher stirring than 300 rpm, for 2 h and 0.5 g of biocatalyst.
Conclusions: These obtained results have proven a very good efficiency of the biocatalyst in the studied selective
process. Furthermore, it was allowed sixteen times the successive reuse of the biocatalyst with good performance