Immunohistochemical study of macrophage and cytokine dynamics in the gut of scrapie-infected mice
Autor
Romero Trevejo, J.L.
Pedrera, Miriam
Sánchez-Cordón, P.J.
Blanco Rodríguez, A.
Bautista Pérez, María José
Gómez-Villamandos, J.C.
Editor
Universidad de MurciaFecha
2010Materia
ScrapieCytokines
Immune response
Gut-associated lymphoid tissues
Pathology
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To study numerical changes in intestinal
macrophages and variations in cytokine production by
immune cells in the intestine, conventional C57BL/6J
mice were orally infected with the Rocky Mountain
Laboratory strain of scrapie. Animals were sacrificed at
different timepoints, and samples were taken and
processed by routine methods for morphological and
immunohistochemical analysis. The results point to a
possible role for macrophages in the uptake and
transport of the infective agent to Peyer’s patches. The
observed increase in macrophage numbers in
subepithelial sites, taken in conjunction with a drop in
tumour necrosis factor-a production at these sites,
suggests a possible secretory inhibition that could be
induced by the disease-associated prion protein (PrPd).
On the other hand, cytokine dynamics indicated the
presence of an impaired Th1-Th2 cell mediated
response, which could facilitate the spread of PrPd to the
central nervous system. Further research is required to
confirm these hypotheses.