Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity
Autor
Régnier, Philippe
Bastias, Jorge
Rodríguez-Ruiz, Violeta
Caballero-Casero, Noelia
Caballo Linares, Carmen
Sicilia Criado, María Dolores
Fuentes, Axelle
Maire, Murielle
Crepin, Michel
Letourneur, Didier
Gueguen, Virginie
Rubio Bravo, Soledad
Pavon-Djavid, Graciela
Editor
MDPIFecha
2015Materia
AstaxanthinAntioxidants
Haematococcus pluvialis
Oxidative stress
HUVEC
TEAC
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Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of
intracellular oxidative stress. The aim of the study was to compare the antioxidant activity
of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae
strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent
or supercritical extraction methods. UV, HPLC-DAD and (HPLC-(atmospheric pressure
chemical ionization (APCI)+)/ion trap-MS) characterizations of both natural extracts
showed similar compositions of carotenoids, but different percentages in free astaxanthin
and its ester derivatives. The Trolox equivalent antioxidant capacity (TEAC) assay showed
that natural extracts containing esters displayed stronger antioxidant activities than free
astaxanthin. Their antioxidant capacities to inhibit intracellular oxidative stress were then evaluated on HUVEC cells. The intracellular antioxidant activity in natural extracts was
approximately 90-times higher than synthetic astaxanthin (5 μM). No modification, neither
in the morphology nor in the viability, of vascular human cells was observed by in vitro
biocompatibility study up to 10 μM astaxanthin concentrations. Therefore, these results
revealed the therapeutic potential of the natural extracts in vascular human cell protection
against oxidative stress without toxicity, which could be exploited in prevention and/or
treatment of cardiovascular diseases