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Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster
dc.contributor.author | Sáez, Lara P. | |
dc.contributor.author | Cabello, Purificación | |
dc.contributor.author | Ibáñez, María Isabel | |
dc.contributor.author | Luque-Almagro, Víctor Manuel | |
dc.contributor.author | Roldán, María Dolores | |
dc.contributor.author | Moreno-Vivián, Conrado | |
dc.date.accessioned | 2019-06-20T07:32:36Z | |
dc.date.available | 2019-06-20T07:32:36Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | http://hdl.handle.net/10396/18758 | |
dc.description.abstract | The alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 can grow with cyanate, cyanide, or cyanide-containing industrial residues as the sole nitrogen source, but the assimilation of cyanide and cyanate takes place through independent pathways. Therefore, cyanide degradation involves a chemical reaction between cyanide and oxaloacetate to form a nitrile that is hydrolyzed to ammonium by the nitrilase NitC, whereas cyanate assimilation requires a cyanase that catalyzes cyanate decomposition to ammonium and carbon dioxide. The P. pseudoalcaligenes CECT5344 cynFABDS gene cluster codes for the putative transcriptional regulator CynF, the ABC-type cyanate transporter CynABD, and the cyanase CynS. In this study, transcriptional analysis revealed that the structural cynABDS genes constitute a single transcriptional unit, which was induced by cyanate and repressed by ammonium. Mutational characterization of the cyn genes indicated that CynF was essential for cynABDS gene expression and that nitrate/nitrite transporters may be involved in cyanate uptake, in addition to the CynABD transport system. Biodegradation of hazardous jewelry wastewater containing high amounts of cyanide and metals was achieved in a batch reactor operating at an alkaline pH after chemical treatment with hydrogen peroxide to oxidize cyanide to cyanate. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.source | International Journal of Molecular Sciences 20(12), 3008 (2019) | es_ES |
dc.subject | Pseudomonas pseudoalcaligenes | es_ES |
dc.subject | Cyanate | es_ES |
dc.subject | Cyanase | es_ES |
dc.subject | Cyanide | es_ES |
dc.subject | Jewelry residue | es_ES |
dc.subject | Nitrate | es_ES |
dc.subject | Nitrite | es_ES |
dc.title | Cyanate Assimilation by the Alkaliphilic Cyanide-Degrading Bacterium Pseudomonas pseudoalcaligenes CECT5344: Mutational Analysis of the cyn Gene Cluster | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.3390/ijms20123008 | es_ES |
dc.relation.projectID | Gobierno de España. RTI2018-099573-B-100 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |