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dc.contributor.authorHidalgo, Manuel
dc.contributor.authorDíaz-Jiménez, María
dc.contributor.authorConsuegra, César
dc.contributor.authorPereira, Blasa
dc.contributor.authorDorado, Jesús
dc.date.accessioned2020-08-20T13:49:16Z
dc.date.available2020-08-20T13:49:16Z
dc.date.issued2020
dc.identifier.urihttp://hdl.handle.net/10396/20410
dc.description.abstractVitrification by direct exposure of sperm to liquid nitrogen is increasing in popularity as an alternative to conventional freezing. In this study, the effect of permeable cryoprotectant agents for donkey sperm vitrification was compared to an extender containing non-permeable cryoprotectants. First, three different concentrations of sucrose (0.1, 0.2, and 0.3 molar, M) and bovine serum albumin, BSA (1, 5, and 10%) were compared. Secondly, the concentration of non-permeable agents producing the most desirable results was compared to an extender containing glycerol as permeable agent. Vitrification was performed by dropping 30 μL of sperm suspension directly into LN2 and warming at 42 °C. Sperm motility (total, TM; and progressive, PM) and plasma membrane integrity, PMI (mean ± SEM) were statistically compared between treatments. Sucrose 0.1 M showed a significantly higher percentage of total sperm motility (21.67 ± 9.22%) than sucrose 0.2 M (14.16 ± 4.50%) and 0.3 M (8.58 ± 6.22%); and no differences were found in comparison to the control (19.71 ± 10.16%). Vitrification with sucrose 0.1 M or BSA 5% obtained similar results for TM (21.67 ± 9.22% vs. 19.93 ± 9.93%), PM (13.42 ± 6.85% vs. 12.54 ± 6.37%) and PMI (40.90 ± 13.51% vs. 37.09 ± 14.28); but both showed higher percentages than glycerol (TM = 9.71 ± 4.19%; PM = 5.47 ± 3.17%; PMI = 28.48 ± 15.55%). In conclusion, donkey sperm vitrification in spheres using non-permeable cryoprotectants exhibited better sperm motility and viability parameters after warming than sperm vitrification using extenders containing permeable cryoprotectants.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/4.0/es_ES
dc.sourceAnimals 10(9), 1462 (2020)es_ES
dc.subjectDonkeyes_ES
dc.subjectSpermes_ES
dc.subjectVitrificationes_ES
dc.subjectSphereses_ES
dc.subjectSucrosees_ES
dc.subjectBSAes_ES
dc.subjectGlyceroles_ES
dc.titleVitrification of Donkey Sperm: Is It Better Using Permeable Cryoprotectants?es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3390/ani10091462es_ES
dc.relation.projectIDGobierno de España. AGL-2013-42726-Res_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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