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Measuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals
dc.contributor.author | Castillo‐Peinado, Laura de los Santos | |
dc.contributor.author | Calderón Santiago, Mónica | |
dc.contributor.author | Herrera-Martínez, Aura D. | |
dc.contributor.author | León-Idougourram, Soraya | |
dc.contributor.author | Gálvez-Moreno, M. Ángeles | |
dc.contributor.author | Sánchez‐Cano, Rafael Luis | |
dc.contributor.author | Bouillon, Roger | |
dc.contributor.author | Quesada Gómez, José Manuel | |
dc.contributor.author | Priego-Capote, Feliciano | |
dc.date.accessioned | 2022-06-06T12:19:26Z | |
dc.date.available | 2022-06-06T12:19:26Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://hdl.handle.net/10396/23104 | |
dc.description.abstract | The main branch of vitamin D3 metabolism involves several hydroxylation reactions to obtain mono-, di- and trihydroxylated metabolites, including the circulating and active forms—25(OH)D3 and 1,25(OH)2D3, respectively. However, most clinical trials strictly target the determination of 25(OH)D3 to offer a view of the metabolic status of vitamin D3. Due to the growing interest in expanding this restricted view, we have developed a method for measuring vitamin D3 metabolism by determination of vitamin D3, 25(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,24,25(OH)3D3 in human plasma. The method was based on SPE–LC–MS/MS with a large volume injection of human plasma (240 µL). Detection of di- and trihydroxymetabolites, found at the picogram per milliliter level, was attained by the combined action of high preconcentration and clean-up effects. The method allows obtaining information about ratios such as the known vitamin D metabolite ratio (24,25(OH)2D3/25(OH)D3), which can provide complementary views of vitamin D3 metabolic status. The method was applied to a cohort of obese patients and a reference cohort of healthy volunteers to find metabolic correlations between target analytes as well as differences as a function of vitamin D levels within and between cohorts. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | https://creativecommons.org/licenses/by/4.0/ | es_ES |
dc.source | Separations 9(6), 141 (2022) | es_ES |
dc.subject | Vitamin D deficiency | es_ES |
dc.subject | Vitamin D3 | es_ES |
dc.subject | 1,25‐dihydroxyvitamin D3 | es_ES |
dc.subject | 24,25‐dihydroxyvitamin D3 | es_ES |
dc.subject | 1,24,25‐trihydroxyvitamin D3 | es_ES |
dc.subject | SPE–LC–MS/MS | es_ES |
dc.subject | Plasma | es_ES |
dc.title | Measuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individuals | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/separations9060141 | es_ES |
dc.relation.projectID | Gobierno de España. PID2019‐111373RB‐I00 | es_ES |
dc.relation.projectID | Junta de Andalucía. PI‐0038/2019 | es_ES |
dc.relation.projectID | Junta de Andalucía. BIO‐0139 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |