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dc.contributor.authorCastillo‐Peinado, Laura de los Santos
dc.contributor.authorCalderón Santiago, Mónica
dc.contributor.authorHerrera-Martínez, Aura D.
dc.contributor.authorLeón-Idougourram, Soraya
dc.contributor.authorGálvez-Moreno, M. Ángeles
dc.contributor.authorSánchez‐Cano, Rafael Luis
dc.contributor.authorBouillon, Roger
dc.contributor.authorQuesada Gómez, José Manuel
dc.contributor.authorPriego-Capote, Feliciano
dc.date.accessioned2022-06-06T12:19:26Z
dc.date.available2022-06-06T12:19:26Z
dc.date.issued2022
dc.identifier.urihttp://hdl.handle.net/10396/23104
dc.description.abstractThe main branch of vitamin D3 metabolism involves several hydroxylation reactions to obtain mono-, di- and trihydroxylated metabolites, including the circulating and active forms—25(OH)D3 and 1,25(OH)2D3, respectively. However, most clinical trials strictly target the determination of 25(OH)D3 to offer a view of the metabolic status of vitamin D3. Due to the growing interest in expanding this restricted view, we have developed a method for measuring vitamin D3 metabolism by determination of vitamin D3, 25(OH)D3, 24,25(OH)2D3, 1,25(OH)2D3 and 1,24,25(OH)3D3 in human plasma. The method was based on SPE–LC–MS/MS with a large volume injection of human plasma (240 µL). Detection of di- and trihydroxymetabolites, found at the picogram per milliliter level, was attained by the combined action of high preconcentration and clean-up effects. The method allows obtaining information about ratios such as the known vitamin D metabolite ratio (24,25(OH)2D3/25(OH)D3), which can provide complementary views of vitamin D3 metabolic status. The method was applied to a cohort of obese patients and a reference cohort of healthy volunteers to find metabolic correlations between target analytes as well as differences as a function of vitamin D levels within and between cohorts.es_ES
dc.format.mimetypeapplication/pdfes_ES
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightshttps://creativecommons.org/licenses/by/4.0/es_ES
dc.sourceSeparations 9(6), 141 (2022)es_ES
dc.subjectVitamin D deficiencyes_ES
dc.subjectVitamin D3es_ES
dc.subject1,25‐dihydroxyvitamin D3es_ES
dc.subject24,25‐dihydroxyvitamin D3es_ES
dc.subject1,24,25‐trihydroxyvitamin D3es_ES
dc.subjectSPE–LC–MS/MSes_ES
dc.subjectPlasmaes_ES
dc.titleMeasuring Vitamin D3 Metabolic Status, Comparison between Vitamin D Deficient and Sufficient Individualses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttps://doi.org/10.3390/separations9060141es_ES
dc.relation.projectIDGobierno de España. PID2019‐111373RB‐I00es_ES
dc.relation.projectIDJunta de Andalucía. PI‐0038/2019es_ES
dc.relation.projectIDJunta de Andalucía. BIO‐0139es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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