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Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata)
dc.contributor.author | Jurado, Juan | |
dc.contributor.author | Fuentes-Almagro, Carlos | |
dc.contributor.author | Guardiola, Francisco Antonio | |
dc.contributor.author | Cuesta, Alberto | |
dc.contributor.author | Esteban, Mª Ángeles | |
dc.contributor.author | Prieto-Álamo, M. José | |
dc.date.accessioned | 2024-01-23T19:59:28Z | |
dc.date.available | 2024-01-23T19:59:28Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1874-3919 | |
dc.identifier.uri | http://hdl.handle.net/10396/26698 | |
dc.description.abstract | Fish skin mucus is the first line of defense against infections and it discriminates between pathogenic and commensal bacterial strains. Mucus composition varies amongst fish species and is influenced by endogenous and exogenous factors. This study describes the first proteome map of the epidermal mucus of farmed gilthead seabream (Sparus aurata). We used an integrative proteomic approach by combining a label-free procedure (LC-MS/MS) with the classical 2-DE-PMF-MS/MS methodology. The identified mucosal proteins were clustered in four groups according to their biological functions. Structural proteins (actins, keratins, tubulins, tropomyosin, cofilin-2 and filamin-A) and metabolic proteins (ribosomal proteins, proteasomal subunits, NACA, VCP, histones, NDPK, transferrin, glycolytic enzymes, ATP synthase components, beta-globin, Apo-A1 and FABP7) were the best represented functional categories. We also found proteins involved in stress response (WAP65, HSPC70, Cu,Zn-SOD, and PRDX1 and PRDX2) and signal transduction (PP2A 65kDa regulatory subunit, 14-3-3 protein beta/alpha, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, RhoGDI and PEBP1). Most of the identified proteins address different aspects of the innate immune response. Additionally, we analyzed bacterial peptides identified in the skin mucus of healthy S. aurata. These results revealed that genera belonging to the Lactobacillales order constitute the most abundant microorganism populations in this habitat. | es_ES |
dc.format.mimetype | application/pdf | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/4.0/ | es_ES |
dc.source | Jurado, J., Fuentes-Almagro, C. A., Guardiola, F. A., Cuesta, A., Esteban, M. Á., & Prieto-Álamo, M. J. (2015). Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata). Journal of proteomics, 120, 21–34. https://doi.org/10.1016/j.jprot.2015.02.019 | es_ES |
dc.subject | Skin mucus | es_ES |
dc.subject | Gilthead seabream (Sparus aurata L.) | es_ES |
dc.subject | Teleost | es_ES |
dc.subject | Proteome | es_ES |
dc.subject | Microbiota | es_ES |
dc.title | Proteomic profile of the skin mucus of farmed gilthead seabream (Sparus aurata) | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.jprot.2015.02.019 | es_ES |
dc.relation.projectID | Gobierno de España. AGL2011-30381-C03-03 | es_ES |
dc.relation.projectID | Gobierno de España. AGL2011-30381-C03-01 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |