Listar Departamento de Bioquímica y Biología Molecular por autor "Repetto, Guillermo"
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Direct Determination of Glutathione Reductase in Cells Cultured in Microtitre Plates as a Biomarker for Oxidative Stress
García-Alfonso, Concepción; Sanz, Pilar; Repetto, Guillermo; Repetto, Manuel; López-Barea, Juan (Fund for the Replacement of Animals in Medical Experiments, 1995)A new method was developed for the direct determination of glutathione reductase (GOR) activity in Vero cells cultured in microtitre plates, avoiding cell-free extract preparation. The cells in each well were washed twice ... -
Direct Determination of Glutathione S-transferase and Glucose-6-phosphate Dehydrogenase Activities in Cells Cultured in Microtitre Plates as Biomarkers for Oxidative Stress
García-Alfonso, Concepción; Repetto, Guillermo; Sanz, Pilar; Repetto, Manuel; López-Barea, Juan (Fund for the Replacement of Animals in Medical Experiments, 1998)The enzymes glutathione S,-transferase (GST) and glucose-6-phosphate dehydrogenase (G-6PDH) are implicated in the defence against oxidative stress. GST is mainly involved in the conjugation of electrophilic compounds ...