Listar por autor "6d563714-7a85-43e0-baeb-12891cf8d320"
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Evaluation of candidate reference genes for expression studies in Pisum sativum under different experimental conditions
Die, Jose V.; Román, Belén; Nadal, Salvador; González-Verdejo, Clara I. (Springer, 2010)Real-time reverse transcriptase quantitative polymerase chain reaction is the most accurate measure of gene expression in biological systems. The data is analyzed through a process called normalization. Internal standards ... -
Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection
Die, Jose V.; Román, Belén; Nadal, Salvador; Dita, Miguel; González-Verdejo, Clara I. (CSIRO, 2009)The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental ... -
Gene expression analysis of molecular mechanisms of defense induced in Medicago truncatula parasitized by Orobanche crenata
Die, Jose V.; González-Verdejo, Clara I.; Dita, Miguel; Nadal, Salvador; Román, Belén (Elsevier, 2009)The infection of Medicago truncatula Gaertn. roots with the obligate parasite Orobanche crenata Forsk. is a useful model for studying the molecular events involved in the legumes–parasite interaction. In order to gain ... -
Selection of housekeeping genes for normalization by real-time RT–PCR: Analysis of Or-MYB1 gene expression in Orobanche ramosa development
González-Verdejo, Clara I.; Die, Jose V.; Nadal, Salvador; Jiménez-Marín, Ángeles; Moreno, Mª Teresa; Román, Belén (Elsevier, 2008)Real-time PCR has become the method of choice for accurate and in-depth expression studies of candidate genes. To avoid bias, real-time PCR is referred to one or several internal control genes that should not fluctuate ...