Desarrollo de protocolo alternativo para la determinación de ceras en aceites de oliva mediante extracción en fase sólida y cromatografía de gases

Abstract

The evaluation of the content of waxes and Fatty Acid Ethyl Esters is request both by IOC Trade Standard and by Regulation (EEC) 2568/91 and its further amendments. The official method uses 15 g of silicic acid and elutes several fractions by using huge volumes of dangerous solvent (n-hexane). The study by Milani et. Al (2020) have reported an alternative method that reduces the amount of reagents and uses less toxic solvent such as isooctane by SPE. From Qorteba Internacional S.L. we have decided make a “home validation” (precision, accuracy and uncertainly) to evaluate the capacity of application of this method in our company.