Short communication. In vitro oocyte maturation and fertilization rates in the Spanish Lidia bovine breed
Ver/
Autor
Demyda-Peyrás, S.
Hidalgo, Manuel
Dorado, Jesús
Anter, J.
Género, Enrique Rubén
Moreno-Millán, M.
Editor
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Fecha
2013Materia
Breed effectBullfight
in vitro fertilization
Lidia cattle breed
METS:
Mostrar el registro METSPREMIS:
Mostrar el registro PREMISMetadatos
Mostrar el registro completo del ítemResumen
The Lidia bovine breed is the most successful cattle breed on the Iberian Peninsula, also considered a hallmark of
Spanish tradition and image around the world. The aims of the study were to characterize the oocyte recovery rates
and to evaluate the effect of two standard in vitro maturation protocols on oocyte maturation (cumulus expansion and
nuclear maturation) and fertilization rates after in vitro fertilization in this breed. For this purpose, 261 ovaries from
Lidia cows were processed obtaining 1,125 viable cumulus oocyte complexes (COCs). The oocyte recovery rate obtained
(4.31 viable COCs per ovary) was lower than those described previously in other studied breeds. Maturation rates were
evaluated in two different oocyte maturation media with (M1) and without (M2) hormonal supplementation. The
percentage of COCs with expanded cumulus cells was significantly lower in M1 (74.35%) compared with M2 (82.25%).
Metaphase II (MII) rates (67.75% in M1 and 73.18% in M2) were similar to previous studies in different cattle
populations. M2 significantly improved the percentage of COCs with their cumulus cells expanded (p < 0.01) and
nuclear maturation rates (p < 0.05), but it did not affect the fertilization percentages obtained in this experiment. In
conclusion, our study suggests that oocytes of the Lidia cattle breed can be obtained, matured and fertilized following
standard protocols previously used in other cattle breeds.